This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Joardar, V.
Right arrow Articles by Buell, C. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Joardar, V.
Right arrow Articles by Buell, C. R.

 Previous Article  |  Next Article 

Journal of Bacteriology, September 2005, p. 6488-6498, Vol. 187, No. 18
0021-9193/05/$08.00+0     doi:10.1128/JB.187.18.6488-6498.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Whole-Genome Sequence Analysis of Pseudomonas syringae pv. phaseolicola 1448A Reveals Divergence among Pathovars in Genes Involved in Virulence and Transposition{dagger}

Vinita Joardar,1 Magdalen Lindeberg,2 Robert W. Jackson,3 Jeremy Selengut,1 Robert Dodson,1 Lauren M. Brinkac,1 Sean C. Daugherty,1 Robert DeBoy,1 A. Scott Durkin,1 Michelle Gwinn Giglio,1 Ramana Madupu,1 William C. Nelson,1 M. J. Rosovitz,1 Steven Sullivan,1 Jonathan Crabtree,1 Todd Creasy,1 Tanja Davidsen,1 Dan H. Haft,1 Nikhat Zafar,1 Liwei Zhou,1 Rebecca Halpin,1 Tara Holley,1 Hoda Khouri,1 Tamara Feldblyum,1 Owen White,1 Claire M. Fraser,1 Arun K. Chatterjee,4 Sam Cartinhour,5 David J. Schneider,5 John Mansfield,6 Alan Collmer,2 and C. Robin Buell1*

The Institute for Genomic Research, 9712 Medical Center Dr., Rockville, Maryland 20850,1 Department of Plant Pathology, Cornell University, Ithaca, New York 14853,2 Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath, BA2 7AY United Kingdom,3 Department of Plant Microbiology and Pathology, University of Missouri, Columbia, Missouri 65211,4 U.S. Department of Agriculture Agricultural Research Service, Ithaca, New York 14853-2901,5 Division of Biology, Imperial College, Wye Campus, Wye, Ashford, Kent TN25 5AH, United Kingdom6

Received 15 April 2005/ Accepted 21 June 2005

Pseudomonas syringae pv. phaseolicola, a gram-negative bacterial plant pathogen, is the causal agent of halo blight of bean. In this study, we report on the genome sequence of P. syringae pv. phaseolicola isolate 1448A, which encodes 5,353 open reading frames (ORFs) on one circular chromosome (5,928,787 bp) and two plasmids (131,950 bp and 51,711 bp). Comparative analyses with a phylogenetically divergent pathovar, P. syringae pv. tomato DC3000, revealed a strong degree of conservation at the gene and genome levels. In total, 4,133 ORFs were identified as putative orthologs in these two pathovars using a reciprocal best-hit method, with 3,941 ORFs present in conserved, syntenic blocks. Although these two pathovars are highly similar at the physiological level, they have distinct host ranges; 1448A causes disease in beans, and DC3000 is pathogenic on tomato and Arabidopsis. Examination of the complement of ORFs encoding virulence, fitness, and survival factors revealed a substantial, but not complete, overlap between these two pathovars. Another distinguishing feature between the two pathovars is their distinctive sets of transposable elements. With access to a fifth complete pseudomonad genome sequence, we were able to identify 3,567 ORFs that likely comprise the core Pseudomonas genome and 365 ORFs that are P. syringae specific.

* Corresponding author. Mailing address: The Institute for Genomic Research, 9712 Medical Center Dr., Rockville, MD 20850. Phone: (301) 795-7558. Fax: (301) 838-0208. E-mail: rbuell{at}tigr.org.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, September 2005, p. 6488-6498, Vol. 187, No. 18
0021-9193/05/$08.00+0     doi:10.1128/JB.187.18.6488-6498.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Daddaoua, A., Krell, T., Ramos, J.-L. (2009). Regulation of Glucose Metabolism in Pseudomonas: THE PHOSPHORYLATIVE BRANCH AND ENTNER-DOUDOROFF ENZYMES ARE REGULATED BY A REPRESSOR CONTAINING A SUGAR ISOMERASE DOMAIN. J. Biol. Chem. 284: 21360-21368 [Abstract] [Full Text]  
  • Collmer, A., Schneider, D. J., Lindeberg, M. (2009). Lifestyles of the Effector Rich: Genome-Enabled Characterization of Bacterial Plant Pathogens. Plant Physiol. 150: 1623-1630 [Full Text]  
  • Wroblewski, T., Caldwell, K. S., Piskurewicz, U., Cavanaugh, K. A., Xu, H., Kozik, A., Ochoa, O., McHale, L. K., Lahre, K., Jelenska, J., Castillo, J. A., Blumenthal, D., Vinatzer, B. A., Greenberg, J. T., Michelmore, R. W. (2009). Comparative Large-Scale Analysis of Interactions between Several Crop Species and the Effector Repertoires from Multiple Pathovars of Pseudomonas and Ralstonia. Plant Physiol. 150: 1733-1749 [Abstract] [Full Text]  
  • Berti, A. D., Thomas, M. G. (2009). Analysis of Achromobactin Biosynthesis by Pseudomonas syringae pv. syringae B728a. J. Bacteriol. 191: 4594-4604 [Abstract] [Full Text]  
  • Moynihan, J. A., Morrissey, J. P., Coppoolse, E. R., Stiekema, W. J., O'Gara, F., Boyd, E. F. (2009). Evolutionary History of the phl Gene Cluster in the Plant-Associated Bacterium Pseudomonas fluorescens. Appl. Environ. Microbiol. 75: 2122-2131 [Abstract] [Full Text]  
  • Reinhardt, J. A., Baltrus, D. A., Nishimura, M. T., Jeck, W. R., Jones, C. D., Dangl, J. L. (2009). De novo assembly using low-coverage short read sequence data from the rice pathogen Pseudomonas syringae pv. oryzae. Genome Res 19: 294-305 [Abstract] [Full Text]  
  • Kiely, P. D., O'Callaghan, J., Abbas, A., O'Gara, F. (2008). Genetic analysis of genes involved in dipeptide metabolism and cytotoxicity in Pseudomonas aeruginosa PAO1. Microbiology 154: 2209-2218 [Abstract] [Full Text]  
  • Stoitsova, S. O., Braun, Y., Ullrich, M. S., Weingart, H. (2008). Characterization of the RND-Type Multidrug Efflux Pump MexAB-OprM of the Plant Pathogen Pseudomonas syringae. Appl. Environ. Microbiol. 74: 3387-3393 [Abstract] [Full Text]  
  • Yan, S., Liu, H., Mohr, T. J., Jenrette, J., Chiodini, R., Zaccardelli, M., Setubal, J. C., Vinatzer, B. A. (2008). Role of Recombination in the Evolution of the Model Plant Pathogen Pseudomonas syringae pv. tomato DC3000, a Very Atypical Tomato Strain. Appl. Environ. Microbiol. 74: 3171-3181 [Abstract] [Full Text]  
  • Mohr, T. J., Liu, H., Yan, S., Morris, C. E., Castillo, J. A., Jelenska, J., Vinatzer, B. A. (2008). Naturally Occurring Nonpathogenic Isolates of the Plant Pathogen Pseudomonas syringae Lack a Type III Secretion System and Effector Gene Orthologues. J. Bacteriol. 190: 2858-2870 [Abstract] [Full Text]  
  • Hoopman, T. C., Wang, W., Brautigam, C. A., Sedillo, J. L., Reilly, T. J., Hansen, E. J. (2008). Moraxella catarrhalis Synthesizes an Autotransporter That Is an Acid Phosphatase. J. Bacteriol. 190: 1459-1472 [Abstract] [Full Text]  
  • Perez-Martinez, I., Zhao, Y., Murillo, J., Sundin, G. W., Ramos, C. (2008). Global Genomic Analysis of Pseudomonas savastanoi pv. savastanoi Plasmids. J. Bacteriol. 190: 625-635 [Abstract] [Full Text]  
  • Nuccio, S.-P., Baumler, A. J. (2007). Evolution of the Chaperone/Usher Assembly Pathway: Fimbrial Classification Goes Greek. Microbiol. Mol. Biol. Rev. 71: 551-575 [Abstract] [Full Text]  
  • Berti, A. D., Greve, N. J., Christensen, Q. H., Thomas, M. G. (2007). Identification of a Biosynthetic Gene Cluster and the Six Associated Lipopeptides Involved in Swarming Motility of Pseudomonas syringae pv. tomato DC3000. J. Bacteriol. 189: 6312-6323 [Abstract] [Full Text]  
  • Chatterjee, A., Cui, Y., Hasegawa, H., Chatterjee, A. K. (2007). PsrA, the Pseudomonas Sigma Regulator, Controls Regulators of Epiphytic Fitness, Quorum-Sensing Signals, and Plant Interactions in Pseudomonas syringae pv. tomato Strain DC3000. Appl. Environ. Microbiol. 73: 3684-3694 [Abstract] [Full Text]  
  • Bantinaki, E., Kassen, R., Knight, C. G., Robinson, Z., Spiers, A. J., Rainey, P. B. (2007). Adaptive Divergence in Experimental Populations of Pseudomonas fluorescens. III. Mutational Origins of Wrinkly Spreader Diversity. Genetics 176: 441-453 [Abstract] [Full Text]  
  • Aguilera, S., Lopez-Lopez, K., Nieto, Y., Garciduenas-Pina, R., Hernandez-Guzman, G., Hernandez-Flores, J. L., Murillo, J., Alvarez-Morales, A. (2007). Functional Characterization of the Gene Cluster from Pseudomonas syringae pv. phaseolicola NPS3121 Involved in Synthesis of Phaseolotoxin. J. Bacteriol. 189: 2834-2843 [Abstract] [Full Text]  
  • Schluter, A., Szczepanowski, R., Kurz, N., Schneiker, S., Krahn, I., Puhler, A. (2007). Erythromycin Resistance-Conferring Plasmid pRSB105, Isolated from a Sewage Treatment Plant, Harbors a New Macrolide Resistance Determinant, an Integron-Containing Tn402-Like Element, and a Large Region of Unknown Function. Appl. Environ. Microbiol. 73: 1952-1960 [Abstract] [Full Text]  
  • Alavi, S. M., Poussier, S., Manceau, C. (2007). Characterization of ISXax1, a Novel Insertion Sequence Restricted to Xanthomonas axonopodis pv. phaseoli (Variants fuscans and non-fuscans) and Xanthomonas axonopodis pv. vesicatoria. Appl. Environ. Microbiol. 73: 1678-1682 [Abstract] [Full Text]  
  • Ma, Z., Smith, J. J., Zhao, Y., Jackson, R. W., Arnold, D. L., Murillo, J., Sundin, G. W. (2007). Phylogenetic Analysis of the pPT23A Plasmid Family of Pseudomonas syringae. Appl. Environ. Microbiol. 73: 1287-1295 [Abstract] [Full Text]  
  • Angot, A., Peeters, N., Lechner, E., Vailleau, F., Baud, C., Gentzbittel, L., Sartorel, E., Genschik, P., Boucher, C., Genin, S. (2006). Ralstonia solanacearum requires F-box-like domain-containing type III effectors to promote disease on several host plants. Proc. Natl. Acad. Sci. USA 103: 14620-14625 [Abstract] [Full Text]  
  • Fu, Z. Q., Guo, M., Alfano, J. R. (2006). Pseudomonas syringae HrpJ Is a Type III Secreted Protein That Is Required for Plant Pathogenesis, Injection of Effectors, and Secretion of the HrpZ1 Harpin.. J. Bacteriol. 188: 6060-6069 [Abstract] [Full Text]  
  • Bultreys, A., Gheysen, I., de Hoffmann, E. (2006). Yersiniabactin Production by Pseudomonas syringae and Escherichia coli, and Description of a Second Yersiniabactin Locus Evolutionary Group.. Appl. Environ. Microbiol. 72: 3814-3825 [Abstract] [Full Text]  
  • Araki, H., Tian, D., Goss, E. M., Jakob, K., Halldorsdottir, S. S., Kreitman, M., Bergelson, J. (2006). Presence/absence polymorphism for alternative pathogenicity islands in Pseudomonas viridiflava, a pathogen of Arabidopsis. Proc. Natl. Acad. Sci. USA 103: 5887-5892 [Abstract] [Full Text]  
  • Champ, P. C., Binnewies, T. T., Nielsen, N., Zinman, G., Kiil, K., Wu, H., Bohlin, J., Ussery, D. W. (2006). Genome update: purine strand bias in 280 bacterial genomes.. Microbiology 152: 579-583 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»