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Journal of Bacteriology, September 2005, p. 6561-6564, Vol. 187, No. 18
0021-9193/05/$08.00+0     doi:10.1128/JB.187.18.6561-6564.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Evolutionary and Diagnostic Implications of Intragenomic Heterogeneity in the 16S rRNA Gene in Aeromonas Strains

Alessia Morandi,^1, Olga Zhaxybayeva,^2, J. Peter Gogarten,² and Joerg Graf^2*

Institute for Infectious Diseases, University of Berne, Berne, Switzerland,¹ Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut²

Received 31 May 2005/ Accepted 7 July 2005

Sequencing 16S rRNA genes (SSU) cloned from Aeromonas strains revealed that strains contained up to six copies differing by 1.5%. The SSU copies from Aeromonas veronii LMG13695 clustered with sequences from four Aeromonas species. These results demonstrate intragenomic heterogeneity of SSU and suggest caution when using SSU to identify aeromonads.

Present address: Servizio Psico-Sociale, Vicolo Scottocorte 4, 6500 Bellinzona, Switzerland.

Present address: Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Nova Scotia, Canada.

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