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Journal of Bacteriology, September 2005, p. 6565-6570, Vol. 187, No. 18
0021-9193/05/$08.00+0     doi:10.1128/JB.187.18.6565-6570.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Deletion of the Gene rpoZ, Encoding the {omega} Subunit of RNA Polymerase, in Mycobacterium smegmatis Results in Fragmentation of the ß' Subunit in the Enzyme Assembly

Renjith Mathew,1 Madhugiri Ramakanth,2 and Dipankar Chatterji1*

Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560012, India,1 Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore, India2

Received 14 April 2005/ Accepted 21 June 2005

A deletion mutation in the gene rpoZ of Mycobacterium smegmatis causes reduced growth rate and a change in colony morphology. During purification of RNA polymerase from the mutant strain, the ß' subunit undergoes fragmentation but the fragments remain associated with the enzyme and maintain it in an active state until the whole destabilized assembly breaks down in the final step of purification. Complementation of the mutant strain with an integrated copy of the wild-type rpoZ brings back the wild-type colony morphology and improves the growth rate and activity of the enzyme, and the integrity of the ß' subunit remains unaffected.

* Corresponding author. Mailing address: Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India. Phone: 91-80- 22932836. Fax: 91-80-23600535. E-mail: dipankar{at}mbu.iisc.ernet.in.

Journal of Bacteriology, September 2005, p. 6565-6570, Vol. 187, No. 18
0021-9193/05/$08.00+0     doi:10.1128/JB.187.18.6565-6570.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Chowdhury, R. P., Gupta, S., Chatterji, D. (2007). Identification and Characterization of the dps Promoter of Mycobacterium smegmatis: Promoter Recognition by Stress-Specific Extracytoplasmic Function Sigma Factors {sigma}H and {sigma}F. J. Bacteriol. 189: 8973-8981 [Abstract] [Full Text]  
  • Mathew, R., Mukherjee, R., Balachandar, R., Chatterji, D. (2006). Deletion of the rpoZ gene, encoding the {omega} subunit of RNA polymerase, results in pleiotropic surface-related phenotypes in Mycobacterium smegmatis. Microbiology 152: 1741-1750 [Abstract] [Full Text]  


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