Journal of Bacteriology, September 2005, p. 6577-6579, Vol. 187, No. 18
0021-9193/05/$08.00+0 doi:10.1128/JB.187.18.6577-6579.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.
The MutS C Terminus Is Essential for Mismatch Repair Activity In Vivo
Melissa A. Calmann,
Anetta Nowosielska, and
M. G. Marinus*
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605
Received 17 May 2005/
Accepted 20 June 2005
An Escherichia coli K-12 strain was constructed with a chromosomal deletion (mutS
800) in the mutS gene that produced the removal of the C-terminal 53 amino acids which are not present in the MutS crystal structure. This strain has a MutS null phenotype for mutation avoidance, antirecombination, and sensitivity to cytotoxic agents in a dam mutant background.
* Corresponding author. Mailing address: Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605. Phone: (508) 856-3330. Fax: (508) 856-2003. E-mail: martin.marinus{at}umassmed.edu.
Journal of Bacteriology, September 2005, p. 6577-6579, Vol. 187, No. 18
0021-9193/05/$08.00+0 doi:10.1128/JB.187.18.6577-6579.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.
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Copyright © 2005 by the American Society for Microbiology. All rights reserved.