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Journal of Bacteriology, September 2005, p. 6577-6579, Vol. 187, No. 18
0021-9193/05/$08.00+0     doi:10.1128/JB.187.18.6577-6579.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

The MutS C Terminus Is Essential for Mismatch Repair Activity In Vivo

Melissa A. Calmann, Anetta Nowosielska, and M. G. Marinus*

Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605

Received 17 May 2005/ Accepted 20 June 2005

An Escherichia coli K-12 strain was constructed with a chromosomal deletion (mutS{Delta}800) in the mutS gene that produced the removal of the C-terminal 53 amino acids which are not present in the MutS crystal structure. This strain has a MutS null phenotype for mutation avoidance, antirecombination, and sensitivity to cytotoxic agents in a dam mutant background.

* Corresponding author. Mailing address: Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605. Phone: (508) 856-3330. Fax: (508) 856-2003. E-mail: martin.marinus{at}umassmed.edu.

Journal of Bacteriology, September 2005, p. 6577-6579, Vol. 187, No. 18
0021-9193/05/$08.00+0     doi:10.1128/JB.187.18.6577-6579.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Mendillo, M. L., Putnam, C. D., Kolodner, R. D. (2007). Escherichia coli MutS Tetramerization Domain Structure Reveals That Stable Dimers but Not Tetramers Are Essential for DNA Mismatch Repair in Vivo. J. Biol. Chem. 282: 16345-16354 [Abstract] [Full Text]  
  • Manelyte, L., Urbanke, C., Giron-Monzon, L., Friedhoff, P. (2006). Structural and functional analysis of the MutS C-terminal tetramerization domain. Nucleic Acids Res 34: 5270-5279 [Abstract] [Full Text]  
  • de Saro, F. J. L., Marinus, M. G., Modrich, P., O'Donnell, M. (2006). The beta Sliding Clamp Binds to Multiple Sites within MutL and MutS. J. Biol. Chem. 281: 14340-14349 [Abstract] [Full Text]  


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