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Journal of Bacteriology, September 2005, p. 6590-6595, Vol. 187, No. 18
0021-9193/05/$08.00+0     doi:10.1128/JB.187.18.6590-6595.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Requirements for Heterologous Production of a Complex Metalloenzyme: the Membrane-Bound [NiFe] Hydrogenase

Oliver Lenz, Andrea Gleiche, Angelika Strack, and Bärbel Friedrich*

Institut für Biologie, Humboldt-Universität zu Berlin, D-10115 Berlin, Germany

Received 14 February 2005/ Accepted 14 June 2005

By taking advantage of the tightly clustered genes for the membrane-bound [NiFe] hydrogenase of Ralstonia eutropha H16, broad-host-range recombinant plasmids were constructed carrying the entire membrane-bound hydrogenase (MBH) operon encompassing 21 genes. We demonstrate that the complex MBH biosynthetic apparatus is actively produced in hydrogenase-free hosts yielding fully assembled and functional MBH protein.

* Corresponding author. Mailing address: Institut für Biologie, Humboldt-Universität zu Berlin, Chausseestraße 117, D-10115 Berlin, Germany. Phone: 49 (0)30 2093 8100. Fax: 49 (0)30 2093 8102. E-mail: baerbel.friedrich{at}rz.hu-berlin.de.

Journal of Bacteriology, September 2005, p. 6590-6595, Vol. 187, No. 18
0021-9193/05/$08.00+0     doi:10.1128/JB.187.18.6590-6595.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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  • Saggu, M., Zebger, I., Ludwig, M., Lenz, O., Friedrich, B., Hildebrandt, P., Lendzian, F. (2009). Spectroscopic Insights into the Oxygen-tolerant Membrane-associated [NiFe] Hydrogenase of Ralstonia eutropha H16. J. Biol. Chem. 284: 16264-16276 [Abstract] [Full Text]  
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  • Vincent, K. A., Cracknell, J. A., Lenz, O., Zebger, I., Friedrich, B., Armstrong, F. A. (2005). From The Cover: Electrocatalytic hydrogen oxidation by an enzyme at high carbon monoxide or oxygen levels. Proc. Natl. Acad. Sci. USA 102: 16951-16954 [Abstract] [Full Text]  


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