Journal of Bacteriology, September 2005, p. 6590-6595, Vol. 187, No. 18
0021-9193/05/$08.00+0 doi:10.1128/JB.187.18.6590-6595.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.
Requirements for Heterologous Production of a Complex Metalloenzyme: the Membrane-Bound [NiFe] Hydrogenase
Oliver Lenz,
Andrea Gleiche,
Angelika Strack, and
Bärbel Friedrich*
Institut für Biologie, Humboldt-Universität zu Berlin, D-10115 Berlin, Germany
Received 14 February 2005/
Accepted 14 June 2005
By taking advantage of the tightly clustered genes for the membrane-bound [NiFe] hydrogenase of Ralstonia eutropha H16, broad-host-range recombinant plasmids were constructed carrying the entire membrane-bound hydrogenase (MBH) operon encompassing 21 genes. We demonstrate that the complex MBH biosynthetic apparatus is actively produced in hydrogenase-free hosts yielding fully assembled and functional MBH protein.
* Corresponding author. Mailing address: Institut für Biologie, Humboldt-Universität zu Berlin, Chausseestraße 117, D-10115 Berlin, Germany. Phone: 49 (0)30 2093 8100. Fax: 49 (0)30 2093 8102. E-mail: baerbel.friedrich{at}rz.hu-berlin.de.
Journal of Bacteriology, September 2005, p. 6590-6595, Vol. 187, No. 18
0021-9193/05/$08.00+0 doi:10.1128/JB.187.18.6590-6595.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.
This article has been cited by other articles:
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Copyright © 2005 by the American Society for Microbiology. All rights reserved.