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Journal of Bacteriology, October 2005, p. 6862-6866, Vol. 187, No. 19
0021-9193/05/$08.00+0     doi:10.1128/JB.187.19.6862-6866.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Mutator Phenotype Resulting from DNA Polymerase IV Overproduction in Escherichia coli: Preferential Mutagenesis on the Lagging Strand

Wojciech Kuban,1 Magdalena Banach-Orlowska,1 Malgorzata Bialoskorska,1 Aleksandra Lipowska,1 Roel M. Schaaper,2 Piotr Jonczyk,1 and Iwona J. Fijalkowska1*

Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02 106 Warsaw, Poland,1 Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 277092

Received 30 March 2005/ Accepted 8 July 2005

We investigated the mutator effect resulting from overproduction of Escherichia coli DNA polymerase IV. Using lac mutational targets in the two possible orientations on the chromosome, we observed preferential mutagenesis during lagging strand synthesis. The mutator activity likely results from extension of mismatches produced by polymerase III holoenzyme.

* Corresponding author. Mailing address: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02106 Warsaw, Poland. Phone: (48) 22-592-1113. Fax: (48) 22-658-4636. E-mail: iwonaf{at}ibb.waw.pl.

Journal of Bacteriology, October 2005, p. 6862-6866, Vol. 187, No. 19
0021-9193/05/$08.00+0     doi:10.1128/JB.187.19.6862-6866.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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