This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mashburn, L. M.
Right arrow Articles by Whiteley, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mashburn, L. M.
Right arrow Articles by Whiteley, M.

 Previous Article  |  Next Article 

Journal of Bacteriology, January 2005, p. 554-566, Vol. 187, No. 2
0021-9193/05/$08.00+0     doi:10.1128/JB.187.2.554-566.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Staphylococcus aureus Serves as an Iron Source for Pseudomonas aeruginosa during In Vivo Coculture

Lauren M. Mashburn,1,2 Amy M. Jett,2 Darrin R. Akins,2 and Marvin Whiteley1,2*

Department of Periodontics,1 Department of Microbiology and Immunology, The University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma2

Received 10 August 2004/ Accepted 18 October 2004

Pseudomonas aeruginosa is a gram-negative opportunistic human pathogen often infecting the lungs of individuals with the heritable disease cystic fibrosis and the peritoneum of individuals undergoing continuous ambulatory peritoneal dialysis. Often these infections are not caused by colonization with P. aeruginosa alone but instead by a consortium of pathogenic bacteria. Little is known about growth and persistence of P. aeruginosa in vivo, and less is known about the impact of coinfecting bacteria on P. aeruginosa pathogenesis and physiology. In this study, a rat dialysis membrane peritoneal model was used to evaluate the in vivo transcriptome of P. aeruginosa in monoculture and in coculture with Staphylococcus aureus. Monoculture results indicate that approximately 5% of all P. aeruginosa genes are differentially regulated during growth in vivo compared to in vitro controls. Included in this analysis are genes important for iron acquisition and growth in low-oxygen environments. The presence of S. aureus caused decreased transcription of P. aeruginosa iron-regulated genes during in vivo coculture, indicating that the presence of S. aureus increases usable iron for P. aeruginosa in this environment. We propose a model where P. aeruginosa lyses S. aureus and uses released iron for growth in low-iron environments.

* Corresponding author. Mailing address: Department of Periodontics, The University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104. Phone: (405) 271-5875. Fax: (405) 271-3874. E-mail: marvin-whiteley{at}ouhsc.edu.

Journal of Bacteriology, January 2005, p. 554-566, Vol. 187, No. 2
0021-9193/05/$08.00+0     doi:10.1128/JB.187.2.554-566.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Reid, D. W., Anderson, G. J., Lamont, I. L. (2009). Role of lung iron in determining the bacterial and host struggle in cystic fibrosis. Am. J. Physiol. Lung Cell. Mol. Physiol. 297: L795-L802 [Abstract] [Full Text]  
  • Boulette, M. L., Baynham, P. J., Jorth, P. A., Kukavica-Ibrulj, I., Longoria, A., Barrera, K., Levesque, R. C., Whiteley, M. (2009). Characterization of Alanine Catabolism in Pseudomonas aeruginosa and Its Importance for Proliferation In Vivo. J. Bacteriol. 191: 6329-6334 [Abstract] [Full Text]  
  • Even, S., Charlier, C., Nouaille, S., Ben Zakour, N. L., Cretenet, M., Cousin, F. J., Gautier, M., Cocaign-Bousquet, M., Loubiere, P., Le Loir, Y. (2009). Staphylococcus aureus Virulence Expression Is Impaired by Lactococcus lactis in Mixed Cultures. Appl. Environ. Microbiol. 75: 4459-4472 [Abstract] [Full Text]  
  • Herve-Jimenez, L., Guillouard, I., Guedon, E., Boudebbouze, S., Hols, P., Monnet, V., Maguin, E., Rul, F. (2009). Postgenomic Analysis of Streptococcus thermophilus Cocultivated in Milk with Lactobacillus delbrueckii subsp. bulgaricus: Involvement of Nitrogen, Purine, and Iron Metabolism. Appl. Environ. Microbiol. 75: 2062-2073 [Abstract] [Full Text]  
  • Jakubovics, N. S., Gill, S. R., Iobst, S. E., Vickerman, M. M., Kolenbrander, P. E. (2008). Regulation of Gene Expression in a Mixed-Genus Community: Stabilized Arginine Biosynthesis in Streptococcus gordonii by Coaggregation with Actinomyces naeslundii. J. Bacteriol. 190: 3646-3657 [Abstract] [Full Text]  
  • Goldsworthy, M. J. H. (2008). Gene expression of Pseudomonas aeruginosa and MRSA within a catheter-associated urinary tract infection biofilm model. Bioscience Horizons 1: 28-37 [Abstract] [Full Text]  
  • Palmer, K. L., Aye, L. M., Whiteley, M. (2007). Nutritional Cues Control Pseudomonas aeruginosa Multicellular Behavior in Cystic Fibrosis Sputum. J. Bacteriol. 189: 8079-8087 [Abstract] [Full Text]  
  • Farrow, J. M. III, Pesci, E. C. (2007). Two Distinct Pathways Supply Anthranilate as a Precursor of the Pseudomonas Quinolone Signal. J. Bacteriol. 189: 3425-3433 [Abstract] [Full Text]  
  • Harrison, F. (2007). Microbial ecology of the cystic fibrosis lung. Microbiology 153: 917-923 [Abstract] [Full Text]  
  • Hoffman, L. R., Deziel, E., D'Argenio, D. A., Lepine, F., Emerson, J., McNamara, S., Gibson, R. L., Ramsey, B. W., Miller, S. I. (2006). Selection for Staphylococcus aureus small-colony variants due to growth in the presence of Pseudomonas aeruginosa. Proc. Natl. Acad. Sci. USA 103: 19890-19895 [Abstract] [Full Text]  
  • McNamara, P. J., Proctor, R. A. (2006). Bacterial Interactions and the Microevolution of Cytochrome bd: Implications for Pathogenesis. J. Bacteriol. 188: 7997-7998 [Full Text]  
  • Fang, H., Hedin, G. (2006). Use of Cefoxitin-Based Selective Broth for Improved Detection of Methicillin-Resistant Staphylococcus aureus. J. Clin. Microbiol. 44: 592-594 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»