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Journal of Bacteriology, January 2005, p. 687-696, Vol. 187, No. 2
0021-9193/05/$08.00+0     doi:10.1128/JB.187.2.687-696.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Biochemical Activities of the absA Two-Component System of Streptomyces coelicolor

Nancy L. Sheeler, Susan V. MacMillan, and Justin R. Nodwell*

Department of Biochemistry and Biomedical Sciences, McMaster University Health Sciences Centre, Hamilton, Ontario, Canada

Received 19 August 2004/ Accepted 12 October 2004

The AbsA1 sensor kinase and its cognate response regulator AbsA2 are important regulators of antibiotic synthesis in Streptomyces coelicolor. While certain point mutations in absA1 reduce or eliminate the synthesis of several antibiotics, null mutations in these genes bring about enhanced antibiotic synthesis. We show here that AbsA1, which is unusual in sequence and structure, is both an AbsA2 kinase and an AbsA2~P phosphatase. The half-life of AbsA2~P in solution is 68.6 min, consistent with a role in maintaining a relatively stable state of transcriptional repression or activation. We find that mutations in the absA locus that enhance antibiotic synthesis impair AbsA2 kinase activity and that mutations that repress antibiotic synthesis impair AbsA2~P phosphatase activity. These results support a model in which the phosphorylation state of AbsA2 is determined by the balance of the kinase and phosphatase activities of AbsA1 and where AbsA2~P represses antibiotic biosynthetic genes either directly or indirectly.

* Corresponding author. Mailing address: Department of Biochemistry & Biomedical Sciences, McMaster University Health Sciences Centre, 1200 Main St. W., Hamilton, Ontario, Canada L8N 3Z5. Phone: (905) 525-9140, ext. 27335. Fax: (905) 522-9033. E-mail: nodwellj{at}mcmaster.ca.

Journal of Bacteriology, January 2005, p. 687-696, Vol. 187, No. 2
0021-9193/05/$08.00+0     doi:10.1128/JB.187.2.687-696.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • McKenzie, N. L., Nodwell, J. R. (2009). Transmembrane topology of the AbsA1 sensor kinase of Streptomyces coelicolor. Microbiology 155: 1812-1818 [Abstract] [Full Text]  
  • McKenzie, N. L., Nodwell, J. R. (2007). Phosphorylated AbsA2 Negatively Regulates Antibiotic Production in Streptomyces coelicolor through Interactions with Pathway-Specific Regulatory Gene Promoters. J. Bacteriol. 189: 5284-5292 [Abstract] [Full Text]  
  • Lucana, D. O. d. O., Zou, P., Nierhaus, M., Schrempf, H. (2005). Identification of a novel two-component system SenS/SenR modulating the production of the catalase-peroxidase CpeB and the haem-binding protein HbpS in Streptomyces reticuli. Microbiology 151: 3603-3614 [Abstract] [Full Text]  


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