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Conjugational Genetic Exchange in the Hyperthermophilic Archaeon Sulfolobus acidocaldarius: Intragenic Recombination with Minimal Dependence on Marker Separation

Josh E. Hansen, Amy C. Dill, and Dennis W. Grogan^*

Department of Biological Sciences, University of Cincinnati, Cincinnati, Ohio

Received 8 July 2004/ Accepted 18 October 2004

In Sulfolobus acidocaldarius conjugation assays, recombinant frequency was relatively constant for marker separations from 1,154 bp down to about 50 bp and readily detectable at 10 bp. Three-factor crosses revealed little, if any, genetic linkage over distances of 500 to 600 bp, and large deletion mutants were good donors but poor recipients in matings. The results indicate that most intragenic recombination events occur at one of the mutations, not in the interval between them.

Present address: College of Medicine, University of Cincinnati, Cincinnati, OH 45267.

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