This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sorg, J. A. Articles by Schneewind, O. Search for Related Content PubMed PubMed Citation Articles by Sorg, J. A. Articles by Schneewind, O.

Rejection of Impassable Substrates by Yersinia Type III Secretion Machines

Department of Microbiology, University of Chicago, Chicago, Illinois 60637

Received 4 May 2005/ Accepted 2 August 2005

Type III machines of pathogenic Yersinia spp. transport Yop proteins across the bacterial envelope into host cells. Translational fusions of yopE to the dihydrofolate reductase gene (dhfr) or the ß-galactosidase gene (lacZ) generate hybrid proteins that block type III injection of Yop proteins into host cells, consistent with the canonical view that impassable DHFR and LacZ hybrids jam secretion machines. Mutations in repressors of posttranscriptional gene regulation, Yersinia enterocolitica yscM1 and yscM2 as well as Yersinia pestis lcrQ, relieve the YopE-DHFR-imposed blockade and restore type III injection into host cells. Genetic suppression of the type III blockade does not, however, promote YopE-DHFR secretion. A model is proposed whereby rejection of YopE-DHFR from the secretion pathway inhibits type III gene expression.

This article has been cited by other articles:

• Dawson, J. E., Nicholson, L. K. (2008). Folding kinetics and thermodynamics of Pseudomonas syringae effector protein AvrPto provide insight into translocation via the type III secretion system. Protein Sci. 17: 1109-1119 [Abstract] [Full Text]  
• Sorg, J. A., Blaylock, B., Schneewind, O. (2006). Secretion signal recognition by YscN, the Yersinia type III secretion ATPase. Proc. Natl. Acad. Sci. USA 103: 16490-16495 [Abstract] [Full Text]  
• Blaylock, B., Riordan, K. E., Missiakas, D. M., Schneewind, O. (2006). Characterization of the Yersinia enterocolitica Type III Secretion ATPase YscN and Its Regulator, YscL.. J. Bacteriol. 188: 3525-3534 [Abstract] [Full Text]