This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sorg, J. A.
Right arrow Articles by Schneewind, O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sorg, J. A.
Right arrow Articles by Schneewind, O.

 Previous Article  |  Next Article 

Journal of Bacteriology, October 2005, p. 7090-7102, Vol. 187, No. 20
0021-9193/05/$08.00+0     doi:10.1128/JB.187.20.7090-7102.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Rejection of Impassable Substrates by Yersinia Type III Secretion Machines

Joseph A. Sorg, Nathan C. Miller, Melanie M. Marketon, and Olaf Schneewind*

Department of Microbiology, University of Chicago, Chicago, Illinois 60637

Received 4 May 2005/ Accepted 2 August 2005

Type III machines of pathogenic Yersinia spp. transport Yop proteins across the bacterial envelope into host cells. Translational fusions of yopE to the dihydrofolate reductase gene (dhfr) or the ß-galactosidase gene (lacZ) generate hybrid proteins that block type III injection of Yop proteins into host cells, consistent with the canonical view that impassable DHFR and LacZ hybrids jam secretion machines. Mutations in repressors of posttranscriptional gene regulation, Yersinia enterocolitica yscM1 and yscM2 as well as Yersinia pestis lcrQ, relieve the YopE-DHFR-imposed blockade and restore type III injection into host cells. Genetic suppression of the type III blockade does not, however, promote YopE-DHFR secretion. A model is proposed whereby rejection of YopE-DHFR from the secretion pathway inhibits type III gene expression.

* Corresponding author. Mailing address: Department of Microbiology, 920 E. 58th St., Room 607b, Chicago, IL 60637. Phone: (773) 834-9060. Fax: (773) 834-8150. E-mail: oschnee{at}bsd.uchicago.edu.

Journal of Bacteriology, October 2005, p. 7090-7102, Vol. 187, No. 20
0021-9193/05/$08.00+0     doi:10.1128/JB.187.20.7090-7102.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Dawson, J. E., Seckute, J., De, S., Schueler, S. A., Oswald, A. B., Nicholson, L. K. (2009). Elucidation of a pH-folding switch in the Pseudomonas syringae effector protein AvrPto. Proc. Natl. Acad. Sci. USA 106: 8543-8548 [Abstract] [Full Text]  
  • Riordan, K. E., Sorg, J. A., Berube, B. J., Schneewind, O. (2008). Impassable YscP Substrates and Their Impact on the Yersinia enterocolitica Type III Secretion Pathway. J. Bacteriol. 190: 6204-6216 [Abstract] [Full Text]  
  • Sorg, J. A., Blaylock, B., Schneewind, O. (2006). Secretion signal recognition by YscN, the Yersinia type III secretion ATPase. Proc. Natl. Acad. Sci. USA 103: 16490-16495 [Abstract] [Full Text]  
  • Blaylock, B., Riordan, K. E., Missiakas, D. M., Schneewind, O. (2006). Characterization of the Yersinia enterocolitica Type III Secretion ATPase YscN and Its Regulator, YscL.. J. Bacteriol. 188: 3525-3534 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»