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Journal of Bacteriology, October 2005, p. 7150-7154, Vol. 187, No. 20
0021-9193/05/$08.00+0     doi:10.1128/JB.187.20.7150-7154.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Biosynthesis of Active Bacillus subtilis Urease in the Absence of Known Urease Accessory Proteins

Jong Kyong Kim,1 Scott B. Mulrooney,2 and Robert P. Hausinger1,2,3*

Cell and Molecular Biology Program,1 Department of Microbiology and Molecular Genetics,2 Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824-43203

Received 1 June 2005/ Accepted 27 July 2005

Bacillus subtilis contains urease structural genes but lacks the accessory genes typically required for GTP-dependent incorporation of nickel. Nevertheless, B. subtilis was shown to possess a functional urease, and the recombinant enzyme conferred low levels of nickel-dependent activity to Escherichia coli. Additional investigations of the system lead to the suggestion that B. subtilis may use unidentified accessory proteins for in vivo urease activation.

* Corresponding author. Mailing address: Microbiology and Molecular Genetics, 6193 Biomedical Physical Sciences, Michigan State University, East Lansing, MI 48824-4320. Phone: (517) 355-6463, ext. 1610. Fax: (517) 353-8957. E-mail: hausinge{at}msu.edu.

Journal of Bacteriology, October 2005, p. 7150-7154, Vol. 187, No. 20
0021-9193/05/$08.00+0     doi:10.1128/JB.187.20.7150-7154.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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