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Journal of Bacteriology, October 2005, p. 7150-7154, Vol. 187, No. 20
0021-9193/05/$08.00+0 doi:10.1128/JB.187.20.7150-7154.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.
Cell and Molecular Biology Program,1 Department of Microbiology and Molecular Genetics,2 Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824-43203
Received 1 June 2005/ Accepted 27 July 2005
Bacillus subtilis contains urease structural genes but lacks the accessory genes typically required for GTP-dependent incorporation of nickel. Nevertheless, B. subtilis was shown to possess a functional urease, and the recombinant enzyme conferred low levels of nickel-dependent activity to Escherichia coli. Additional investigations of the system lead to the suggestion that B. subtilis may use unidentified accessory proteins for in vivo urease activation.
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