This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kim, J. K.
Right arrow Articles by Hausinger, R. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kim, J. K.
Right arrow Articles by Hausinger, R. P.

 Previous Article  |  Next Article 

Journal of Bacteriology, October 2005, p. 7150-7154, Vol. 187, No. 20
0021-9193/05/$08.00+0     doi:10.1128/JB.187.20.7150-7154.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Biosynthesis of Active Bacillus subtilis Urease in the Absence of Known Urease Accessory Proteins

Jong Kyong Kim,1 Scott B. Mulrooney,2 and Robert P. Hausinger1,2,3*

Cell and Molecular Biology Program,1 Department of Microbiology and Molecular Genetics,2 Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824-43203

Received 1 June 2005/ Accepted 27 July 2005

Bacillus subtilis contains urease structural genes but lacks the accessory genes typically required for GTP-dependent incorporation of nickel. Nevertheless, B. subtilis was shown to possess a functional urease, and the recombinant enzyme conferred low levels of nickel-dependent activity to Escherichia coli. Additional investigations of the system lead to the suggestion that B. subtilis may use unidentified accessory proteins for in vivo urease activation.

* Corresponding author. Mailing address: Microbiology and Molecular Genetics, 6193 Biomedical Physical Sciences, Michigan State University, East Lansing, MI 48824-4320. Phone: (517) 355-6463, ext. 1610. Fax: (517) 353-8957. E-mail: hausinge{at}msu.edu.

Journal of Bacteriology, October 2005, p. 7150-7154, Vol. 187, No. 20
0021-9193/05/$08.00+0     doi:10.1128/JB.187.20.7150-7154.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Sangari, F. J., Seoane, A., Rodriguez, M. C., Aguero, J., Garcia Lobo, J. M. (2007). Characterization of the Urease Operon of Brucella abortus and Assessment of Its Role in Virulence of the Bacterium. Infect. Immun. 75: 774-780 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»