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Journal of Bacteriology, October 2005, p. 7161-7164, Vol. 187, No. 20
0021-9193/05/$08.00+0     doi:10.1128/JB.187.20.7161-7164.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

The Recombinant Phage Lysin LysK Has a Broad Spectrum of Lytic Activity against Clinically Relevant Staphylococci, Including Methicillin-Resistant Staphylococcus aureus

S. O'Flaherty,1,2,3 A. Coffey,4 W. Meaney,2 G. F. Fitzgerald,3,5 and R. P. Ross1,5*

Dairy Products Research Centre, Teagasc, Moorepark, Fermoy, Co. Cork, Ireland,1 Dairy Production Research Centre, Teagasc, Moorepark, Fermoy, Co. Cork, Ireland,2 Department of Microbiology, University College, Cork, Ireland,3 Department of Biological Sciences, Cork Institute of Technology, Bishopstown, Cork, Ireland,4 Alimentary Pharmabiotic Centre, Cork, Ireland5

Received 29 April 2005/ Accepted 30 July 2005

This study concerns the cloning, characterization, and expression of the lysin (LysK) from staphylococcal phage K in Lactococcus lactis. Lactococcal lysates containing recombinant LysK were found to inhibit a range of different species of staphylococci isolated from bovine and human infection sources, including methicillin-resistant Staphylococcus aureus. LysK thus has potential as an antimicrobial for applications in the prevention and/or treatment of infections caused by staphylococci.

* Corresponding author. Mailing address: R. Paul Ross, Teagasc, Dairy Products Research Centre, Moorepark, Fermoy, Co. Cork, Ireland, Phone: 353-25-42229. Fax: 353-25-42340. E-mail: pross{at}moorepark.teagasc.ie.

Journal of Bacteriology, October 2005, p. 7161-7164, Vol. 187, No. 20
0021-9193/05/$08.00+0     doi:10.1128/JB.187.20.7161-7164.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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