This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Takeuchi, F.
Right arrow Articles by Hiramatsu, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Takeuchi, F.
Right arrow Articles by Hiramatsu, K.

 Previous Article  |  Next Article 

Journal of Bacteriology, November 2005, p. 7292-7308, Vol. 187, No. 21
0021-9193/05/$08.00+0     doi:10.1128/JB.187.21.7292-7308.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Whole-Genome Sequencing of Staphylococcus haemolyticus Uncovers the Extreme Plasticity of Its Genome and the Evolution of Human-Colonizing Staphylococcal Species

Fumihiko Takeuchi,1 Shinya Watanabe,1 Tadashi Baba,1 Harumi Yuzawa,1,{dagger} Teruyo Ito,1 Yuh Morimoto,1 Makoto Kuroda,1,{ddagger} Longzhu Cui,1 Mikio Takahashi,2 Akiho Ankai,2 Shin-ichi Baba,2 Shigehiro Fukui,2 Jean C. Lee,3 and Keiichi Hiramatsu1*

Department of Microbiology and Infection Control Science, Juntendo University, Tokyo 113-8421, Japan,1 National Institute of Technology and Evaluation, Miyagi 983-0833, Japan,2 Channing Laboratory, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts 021153

Received 15 April 2005/ Accepted 4 August 2005

Staphylococcus haemolyticus is an opportunistic bacterial pathogen that colonizes human skin and is remarkable for its highly antibiotic-resistant phenotype. We determined the complete genome sequence of S.haemolyticus to better understand its pathogenicity and evolutionary relatedness to the other staphylococcal species. A large proportion of the open reading frames in the genomes of S.haemolyticus, Staphylococcus aureus, and Staphylococcus epidermidis were conserved in their sequence and order on the chromosome. We identified a region of the bacterial chromosome just downstream of the origin of replication that showed little homology among the species but was conserved among strains within a species. This novel region, designated the "oriC environ," likely contributes to the evolution and differentiation of the staphylococcal species, since it was enriched for species-specific nonessential genes that contribute to the biological features of each staphylococcal species. A comparative analysis of the genomes of S.haemolyticus, S.aureus, and S.epidermidis elucidated differences in their biological and genetic characteristics and pathogenic potentials. We identified as many as 82 insertion sequences in the S.haemolyticus chromosome that probably mediated frequent genomic rearrangements, resulting in phenotypic diversification of the strain. Such rearrangements could have brought genomic plasticity to this species and contributed to its acquisition of antibiotic resistance.

* Corresponding author. Mailing address: 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421 Japan. Phone: 81-3-5802-1040. Fax: 81-3-5684-7830. E-mail: hiram{at}med.juntendo.ac.jp.

{dagger} Present address: Laboratory of Social Genome Science, Department of Medical Genome Science, Graduate School of Frontier Science, University of Tokyo, Tokyo 108-8639, Japan.

{ddagger} Present address: Department of Microbiology, University of Tsukuba, Ibaraki 305-8575, Japan.

Journal of Bacteriology, November 2005, p. 7292-7308, Vol. 187, No. 21
0021-9193/05/$08.00+0     doi:10.1128/JB.187.21.7292-7308.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Chen, L., Mediavilla, J. R., Oliveira, D. C., Willey, B. M., de Lencastre, H., Kreiswirth, B. N. (2009). Multiplex Real-Time PCR for Rapid Staphylococcal Cassette Chromosome mec Typing. J. Clin. Microbiol. 47: 3692-3706 [Abstract] [Full Text]  
  • Llarrull, L. I., Fisher, J. F., Mobashery, S. (2009). Molecular Basis and Phenotype of Methicillin Resistance in Staphylococcus aureus and Insights into New {beta}-Lactams That Meet the Challenge. Antimicrob. Agents Chemother. 53: 4051-4063 [Full Text]  
  • Biswas, L., Biswas, R., Nerz, C., Ohlsen, K., Schlag, M., Schafer, T., Lamkemeyer, T., Ziebandt, A.-K., Hantke, K., Rosenstein, R., Gotz, F. (2009). Role of the Twin-Arginine Translocation Pathway in Staphylococcus. J. Bacteriol. 191: 5921-5929 [Abstract] [Full Text]  
  • Fredheim, E. G. A., Klingenberg, C., Rohde, H., Frankenberger, S., Gaustad, P., Flaegstad, T., Sollid, J. E. (2009). Biofilm Formation by Staphylococcus haemolyticus. J. Clin. Microbiol. 47: 1172-1180 [Abstract] [Full Text]  
  • Baba, T., Kuwahara-Arai, K., Uchiyama, I., Takeuchi, F., Ito, T., Hiramatsu, K. (2009). Complete Genome Sequence of Macrococcus caseolyticus Strain JSCS5402, Reflecting the Ancestral Genome of the Human-Pathogenic Staphylococci. J. Bacteriol. 191: 1180-1190 [Abstract] [Full Text]  
  • Zhou, M., Wu, H. (2009). Glycosylation and biogenesis of a family of serine-rich bacterial adhesins. Microbiology 155: 317-327 [Abstract] [Full Text]  
  • Rosenstein, R., Nerz, C., Biswas, L., Resch, A., Raddatz, G., Schuster, S. C., Gotz, F. (2009). Genome Analysis of the Meat Starter Culture Bacterium Staphylococcus carnosus TM300. Appl. Environ. Microbiol. 75: 811-822 [Abstract] [Full Text]  
  • Shore, A. C., Rossney, A. S., O'Connell, B., Herra, C. M., Sullivan, D. J., Humphreys, H., Coleman, D. C. (2008). Detection of Staphylococcal Cassette Chromosome mec-Associated DNA Segments in Multiresistant Methicillin-Susceptible Staphylococcus aureus (MSSA) and Identification of Staphylococcus epidermidis ccrAB4 in both Methicillin-Resistant S. aureus and MSSA. Antimicrob. Agents Chemother. 52: 4407-4419 [Abstract] [Full Text]  
  • Correa, J. E., De Paulis, A., Predari, S., Sordelli, D. O., Jeric, P. E. (2008). First report of qacG, qacH and qacJ genes in Staphylococcus haemolyticus human clinical isolates. J Antimicrob Chemother 62: 956-960 [Abstract] [Full Text]  
  • Tsuru, T., Kobayashi, I. (2008). Multiple Genome Comparison within a Bacterial Species Reveals a Unit of Evolution Spanning Two Adjacent Genes in a Tandem Paralog Cluster. Mol Biol Evol 25: 2457-2473 [Abstract] [Full Text]  
  • Flahaut, S., Vinogradov, E., Kelley, K. A., Brennan, S., Hiramatsu, K., Lee, J. C. (2008). Structural and Biological Characterization of a Capsular Polysaccharide Produced by Staphylococcus haemolyticus. J. Bacteriol. 190: 1649-1657 [Abstract] [Full Text]  
  • Vernikos, G. S., Parkhill, J. (2008). Resolving the structural features of genomic islands: A machine learning approach. Genome Res 18: 331-342 [Abstract] [Full Text]  
  • Frank, K. L., del Pozo, J. L., Patel, R. (2008). From Clinical Microbiology to Infection Pathogenesis: How Daring To Be Different Works for Staphylococcus lugdunensis. Clin. Microbiol. Rev. 21: 111-133 [Abstract] [Full Text]  
  • Baba, T., Bae, T., Schneewind, O., Takeuchi, F., Hiramatsu, K. (2008). Genome Sequence of Staphylococcus aureus Strain Newman and Comparative Analysis of Staphylococcal Genomes: Polymorphism and Evolution of Two Major Pathogenicity Islands. J. Bacteriol. 190: 300-310 [Abstract] [Full Text]  
  • Dordet-Frisoni, E., Dorchies, G., De Araujo, C., Talon, R., Leroy, S. (2007). Genomic Diversity in Staphylococcus xylosus. Appl. Environ. Microbiol. 73: 7199-7209 [Abstract] [Full Text]  
  • Subedi, A., Ubeda, C., Adhikari, R. P., Penades, J. R., Novick, R. P. (2007). Sequence analysis reveals genetic exchanges and intraspecific spread of SaPI2, a pathogenicity island involved in menstrual toxic shock. Microbiology 153: 3235-3245 [Abstract] [Full Text]  
  • Ubeda, C., Barry, P., Penades, J. R., Novick, R. P. (2007). Inaugural Article: A pathogenicity island replicon in Staphylococcus aureus replicates as an unstable plasmid. Proc. Natl. Acad. Sci. USA 104: 14182-14188 [Abstract] [Full Text]  
  • Sasaki, T., Kikuchi, K., Tanaka, Y., Takahashi, N., Kamata, S., Hiramatsu, K. (2007). Reclassification of Phenotypically Identified Staphylococcus intermedius Strains. J. Clin. Microbiol. 45: 2770-2778 [Abstract] [Full Text]  
  • Fuchs, S., Pane-Farre, J., Kohler, C., Hecker, M., Engelmann, S. (2007). Anaerobic Gene Expression in Staphylococcus aureus. J. Bacteriol. 189: 4275-4289 [Abstract] [Full Text]  
  • Hanssen, A.-M., Sollid, J. U. E. (2007). Multiple Staphylococcal Cassette Chromosomes and Allelic Variants of Cassette Chromosome Recombinases in Staphylococcus aureus and Coagulase-Negative Staphylococci from Norway. Antimicrob. Agents Chemother. 51: 1671-1677 [Abstract] [Full Text]  
  • Watanabe, S., Ito, T., Morimoto, Y., Takeuchi, F., Hiramatsu, K. (2007). Precise Excision and Self-Integration of a Composite Transposon as a Model for Spontaneous Large-Scale Chromosome Inversion/Deletion of the Staphylococcus haemolyticus Clinical Strain JCSC1435. J. Bacteriol. 189: 2921-2925 [Abstract] [Full Text]  
  • Bera, A., Biswas, R., Herbert, S., Gotz, F. (2006). The presence of peptidoglycan o-acetyltransferase in various staphylococcal species correlates with lysozyme resistance and pathogenicity.. Infect. Immun. 74: 4598-4604 [Abstract] [Full Text]  
  • Aguiar-Alves, F., Medeiros, F., Fernandes, O., Gudziki Pereira, R. M., Perdreau-Remington, F., Riley, L. W. (2006). New Staphylococcus aureus Genotyping Method Based on Exotoxin (set) Genes.. J. Clin. Microbiol. 44: 2728-2732 [Abstract] [Full Text]  
  • McCallum, N., Karauzum, H., Getzmann, R., Bischoff, M., Majcherczyk, P., Berger-Bachi, B., Landmann, R. (2006). In Vivo Survival of Teicoplanin-Resistant Staphylococcus aureus and Fitness Cost of Teicoplanin Resistance.. Antimicrob. Agents Chemother. 50: 2352-2360 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»