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Journal of Bacteriology, November 2005, p. 7397-7406, Vol. 187, No. 21
0021-9193/05/$08.00+0     doi:10.1128/JB.187.21.7397-7406.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

{sigma}B Activity in a Staphylococcus aureus hemB Mutant

Maria M. Senn,1 Markus Bischoff,1 Christof von Eiff,2 and Brigitte Berger-Bächi1*

Department of Medical Microbiology, University of Zürich, 8006 Zürich, Switzerland,1 Institute of Medical Microbiology, University Hospital of Münster, 48149 Münster, Germany2

Received 25 April 2005/ Accepted 23 August 2005

Inactivation of hemB in Staphylococcus aureus strain Newman resulted in a small-colony phenotype and was accompanied by an altered expression pattern of global regulators and control of virulence factor production. Transcription profiles followed over 15 h by Northern blot analyses revealed that transcripts of the global regulators arl, rot, sae, sarR, sarS, srr, svrA, and sigB disappeared after the exponential phase and that both agr transcripts were completely absent in the hemB mutant. Apart from a general concentration of transcriptional activity to the exponential phase, premature gene expression was observed for rot, hla, and spa. Nevertheless, reported {sigma}B-dependent transcripts, such as sarC and clfA, were produced throughout the 15-h growth period monitored. The absence of these transcripts in a hemB sigB double mutant demonstrated their dependence on {sigma}B and indicated an unexpected, permanent {sigma}B activity in the hemB mutant. Variations in the extents of the directly {sigma}B-controlled asp23, rsbVW-sigB, and sarC transcripts argue for additional factors modulating {sigma}B activity. This study provides the first extended synopsis of the transcriptional patterns of different regulators over the entire growth cycle in the widely used Newman strain.

* Corresponding author. Mailing address: Department of Medical Microbiology, University of Zürich, Gloriastrasse 32, 8006 Zürich, Switzerland. Phone: 41 44 634 26 50. Fax: 41 44 634 49 06. E-mail: bberger{at}immv.unizh.ch.

Journal of Bacteriology, November 2005, p. 7397-7406, Vol. 187, No. 21
0021-9193/05/$08.00+0     doi:10.1128/JB.187.21.7397-7406.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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