This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rhee, J. E. Articles by Choi, S. H. Search for Related Content PubMed PubMed Citation Articles by Rhee, J. E. Articles by Choi, S. H. Pubmed/NCBI databases Substance via MeSH

 Previous Article  |  Next Article 

Journal of Bacteriology, November 2005, p. 7870-7875, Vol. 187, No. 22
0021-9193/05/$08.00+0     doi:10.1128/JB.187.22.7870-7875.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

CadC Activates pH-Dependent Expression of the Vibrio vulnificus cadBA Operon at a Distance through Direct Binding to an Upstream Region

Jee Eun Rhee,¹ Kun-Soo Kim,² and Sang Ho Choi^1*

Department of Food Science and Technology, School of Agricultural Biotechnology, and Center for Agricultural Biomaterials, Seoul National University, Seoul 151-742,¹ Department of Life Science, Sogang University, Seoul 121-742, South Korea²

Received 12 June 2005/ Accepted 5 September 2005

The Vibrio vulnificus cadBA genes were transcribed as a transcriptional operon by a single promoter, P_cadBA, which was activated by CadC in a pH-dependent manner. A direct interaction between CadC and the P_cadBA DNA was demonstrated, and a CadC binding site centered at –233.5 was mapped by deletion analyses of P_cadBA and confirmed by a DNase I protection assay.

---

* Corresponding author. Mailing address: Department of Food Science and Technology, School of Agricultural Biotechnology, Center for Agricultural Biomaterials, Seoul National University, Seoul 151-742, South Korea. Phone: 82-2-880-4857. Fax: 82-2-873-5095. E-mail: choish{at}snu.ac.kr.

---

Journal of Bacteriology, November 2005, p. 7870-7875, Vol. 187, No. 22
0021-9193/05/$08.00+0     doi:10.1128/JB.187.22.7870-7875.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

This article has been cited by other articles:

• Jones, M. K., Oliver, J. D. (2009). Vibrio vulnificus: Disease and Pathogenesis. Infect. Immun. 77: 1723-1733 [Full Text]  
• Oh, M. H., Lee, S. M., Lee, D. H., Choi, S. H. (2009). Regulation of the Vibrio vulnificus hupA Gene by Temperature Alteration and Cyclic AMP Receptor Protein and Evaluation of Its Role in Virulence. Infect. Immun. 77: 1208-1215 [Abstract] [Full Text]  
• Lee, D. H., Jeong, H. S., Jeong, H. G., Kim, K. M., Kim, H., Choi, S. H. (2008). A Consensus Sequence for Binding of SmcR, a Vibrio vulnificus LuxR Homologue, and Genome-wide Identification of the SmcR Regulon. J. Biol. Chem. 283: 23610-23618 [Abstract] [Full Text]  
• Lee, Y. H., Kim, J. H., Bang, I. S., Park, Y. K. (2008). The Membrane-Bound Transcriptional Regulator CadC Is Activated by Proteolytic Cleavage in Response to Acid Stress. J. Bacteriol. 190: 5120-5126 [Abstract] [Full Text]  
• Jeong, H. G., Choi, S. H. (2008). Evidence that AphB, Essential for the Virulence of Vibrio vulnificus, Is a Global Regulator. J. Bacteriol. 190: 3768-3773 [Abstract] [Full Text]  
• Lee, Y. H., Kim, B. H., Kim, J. H., Yoon, W. S., Bang, S. H., Park, Y. K. (2007). CadC Has a Global Translational Effect during Acid Adaptation in Salmonella enterica Serovar Typhimurium. J. Bacteriol. 189: 2417-2425 [Abstract] [Full Text]  
• Kim, J.-S., Choi, S. H., Lee, J. K. (2006). Lysine Decarboxylase Expression by Vibrio vulnificus Is Induced by SoxR in Response to Superoxide Stress. J. Bacteriol. 188: 8586-8592 [Abstract] [Full Text]  
• Rhee, J. E., Jeong, H. G., Lee, J. H., Choi, S. H. (2006). AphB Influences Acid Tolerance of Vibrio vulnificus by Activating Expression of the Positive Regulator CadC.. J. Bacteriol. 188: 6490-6497 [Abstract] [Full Text]