Journal of Bacteriology, November 2005, p. 7870-7875, Vol. 187, No. 22
0021-9193/05/$08.00+0 doi:10.1128/JB.187.22.7870-7875.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.
CadC Activates pH-Dependent Expression of the Vibrio vulnificus cadBA Operon at a Distance through Direct Binding to an Upstream Region
Jee Eun Rhee,1
Kun-Soo Kim,2 and
Sang Ho Choi1*
Department of Food Science and Technology, School of Agricultural Biotechnology, and Center for Agricultural Biomaterials, Seoul National University, Seoul 151-742,1
Department of Life Science, Sogang University, Seoul 121-742, South Korea2
Received 12 June 2005/
Accepted 5 September 2005
The Vibrio vulnificus cadBA genes were transcribed as a transcriptional operon by a single promoter, PcadBA, which was activated by CadC in a pH-dependent manner. A direct interaction between CadC and the PcadBA DNA was demonstrated, and a CadC binding site centered at 233.5 was mapped by deletion analyses of PcadBA and confirmed by a DNase I protection assay.
* Corresponding author. Mailing address: Department of Food Science and Technology, School of Agricultural Biotechnology, Center for Agricultural Biomaterials, Seoul National University, Seoul 151-742, South Korea. Phone: 82-2-880-4857. Fax: 82-2-873-5095. E-mail: choish{at}snu.ac.kr.
Journal of Bacteriology, November 2005, p. 7870-7875, Vol. 187, No. 22
0021-9193/05/$08.00+0 doi:10.1128/JB.187.22.7870-7875.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.
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Copyright © 2005 by the American Society for Microbiology. All rights reserved.