This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Huang, G. Articles by Tabata, S. Search for Related Content PubMed PubMed Citation Articles by Huang, G. Articles by Tabata, S.

 Previous Article  |  Next Article 

Journal of Bacteriology, February 2005, p. 1114-1123, Vol. 187, No. 3
0021-9193/05/$08.00+0     doi:10.1128/JB.187.3.1114-1123.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Clustered Genes Required for the Synthesis of Heterocyst Envelope Polysaccharide in Anabaena sp. Strain PCC 7120

Guocun Huang ,^1,, Qing Fan,^1, Sigal Lechno-Yossef,¹ Elizabeth Wojciuch,¹ C. Peter Wolk,^1* Takakazu Kaneko,² and Satoshi Tabata²

MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, Michigan,¹ Kazusa DNA Research Institute, Kisarazu, Chiba, Japan²

Received 15 August 2004/ Accepted 22 October 2004

As demonstrated with alr2835 (hepA) and alr2834 (hepC) mutants, heterocysts of Anabaena sp. strain PCC 7120, a filamentous cyanobacterium, must have an envelope polysaccharide layer (the Hep⁺ phenotype) to fix dinitrogen in an oxygen-containing milieu (the Fox⁺ phenotype). Transpositions presumptively responsible for a Fox^– phenotype were localized in open reading frames (ORFs) near hepA and hepC. A mutation in each of nine of these ORFs was complemented by a clone bearing only that single, intact ORF. Heterocysts of the nine mutants were found to lack an envelope polysaccharide layer. Complementation of mutations in alr2832 and alr2840 may have resulted from recombination. However, alr2825, alr2827, alr2831, alr2833, alr2837, alr2839, and alr2841, like hepA and hepC, are required for a Hep⁺ Fox⁺ phenotype.

G.H. and Q.F. contributed equally to the work presented.

Present address: Department of Physiology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390.

This article has been cited by other articles:

• Moslavac, S., Nicolaisen, K., Mirus, O., Al Dehni, F., Pernil, R., Flores, E., Maldener, I., Schleiff, E. (2007). A TolC-Like Protein Is Required for Heterocyst Development in Anabaena sp. Strain PCC 7120. J. Bacteriol. 189: 7887-7895 [Abstract] [Full Text]  
• Wang, Y., Lechno-Yossef, S., Gong, Y., Fan, Q., Wolk, C. P., Xu, X. (2007). Predicted Glycosyl Transferase Genes Located outside the HEP Island Are Required for Formation of Heterocyst Envelope Polysaccharide in Anabaena sp. Strain PCC 7120. J. Bacteriol. 189: 5372-5378 [Abstract] [Full Text]  
• Flores, E., Pernil, R., Muro-Pastor, A. M., Mariscal, V., Maldener, I., Lechno-Yossef, S., Fan, Q., Wolk, C. P., Herrero, A. (2007). Septum-Localized Protein Required for Filament Integrity and Diazotrophy in the Heterocyst-Forming Cyanobacterium Anabaena sp. Strain PCC 7120. J. Bacteriol. 189: 3884-3890 [Abstract] [Full Text]  
• Nayar, A. S., Yamaura, H., Rajagopalan, R., Risser, D. D., Callahan, S. M. (2007). FraG is necessary for filament integrity and heterocyst maturation in the cyanobacterium Anabaena sp. strain PCC 7120. Microbiology 153: 601-607 [Abstract] [Full Text]  
• Lechno-Yossef, S., Fan, Q., Ehira, S., Sato, N., Wolk, C. P. (2006). Mutations in Four Regulatory Genes Have Interrelated Effects on Heterocyst Maturation in Anabaena sp. Strain PCC 7120. J. Bacteriol. 188: 7387-7395 [Abstract] [Full Text]  
• Fan, Q., Lechno-Yossef, S., Ehira, S., Kaneko, T., Ohmori, M., Sato, N., Tabata, S., Wolk, C. P. (2006). Signal Transduction Genes Required for Heterocyst Maturation in Anabaena sp. Strain PCC 7120.. J. Bacteriol. 188: 6688-6693 [Abstract] [Full Text]  
• Wang, Y., Xu, X. (2005). Regulation by hetC of Genes Required for Heterocyst Differentiation and Cell Division in Anabaena sp. Strain PCC 7120. J. Bacteriol. 187: 8489-8493 [Abstract] [Full Text]