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Journal of Bacteriology, February 2005, p. 1192-1195, Vol. 187, No. 3
0021-9193/05/$08.00+0     doi:10.1128/JB.187.3.1192-1195.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Characterization of Phospholipase Activity of the Pseudomonas aeruginosa Type III Cytotoxin, ExoU

Hiromi Sato,1 Jimmy B. Feix,2 Cecilia J. Hillard,3 and Dara W. Frank1*

Department of Microbiology and Molecular Genetics,1 Department of Biophysics,2 Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, Wisconsin3

Received 6 July 2004/ Accepted 2 November 2004

Recombinant ExoU (rExoU) and yeast extract were used to optimize an in vitro phospholipase assay as a basis for identifying the mechanism for enzyme activation and substrate specificity. Our results support a model in which a eukaryotic protein cofactor or complex facilitates the interaction of rExoU with phospholipid substrates.

* Corresponding author. Mailing address: Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, 8701 Watertown Plank Rd., Milwaukee, WI 53226. Phone: (414) 456-8766. Fax: (414) 456-6535. E-mail: frankd{at}mcw.edu.

Journal of Bacteriology, February 2005, p. 1192-1195, Vol. 187, No. 3
0021-9193/05/$08.00+0     doi:10.1128/JB.187.3.1192-1195.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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