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Expression Profiling of Virulence and Pathogenicity Genes of Xanthomonas axonopodis pv. citri

Laboratório de Biotecnologia, Centro APTA Citros Sylvio Moreira, Cordeirópolis, São Paulo,¹ Embrapa Milho e Sorgo, Sete Lagoas, Minas Gerais, Brazil²

Received 14 May 2004/ Accepted 22 October 2004

DNA macroarrays of 279 genes of Xanthomonas axonopodis pv. citri potentially associated with pathogenicity and virulence were used to compare the transcriptional alterations of this bacterium in response to two synthetic media. Data analysis indicated that 31 genes were up-regulated by synthetic medium XVM2, while only 7 genes were repressed. The results suggest that XVM2 could be used as an in vitro system to identify candidate genes involved in pathogenesis of X. axonopodis pv. citri.

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