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Identification and Characterization of Genes Involved in the Downstream Degradation Pathway of -Hexachlorocyclohexane in Sphingomonas paucimobilis UT26

Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University, Sendai,¹ Department of Bioengineering, Nagaoka University of Technology, Kamitomioka, Nagaoka, Niigata, Japan²

Received 25 August 2004/ Accepted 2 November 2004

Sphingomonas paucimobilis UT26 utilizes -hexachlorocyclohexane (-HCH) as a sole source of carbon and energy. In our previous study, we cloned and characterized genes that are involved in the conversion of -HCH to maleylacetate (MA) via chlorohydroquinone (CHQ) in UT26. In this study, we identified and characterized an MA reductase gene, designated linF, that is essential for the utilization of -HCH in UT26. A gene named linEb, whose deduced product showed significant identity to LinE (53%), was located close to linF. LinE is a novel type of ring cleavage dioxygenase that catalyzes the conversion of CHQ to MA. LinEb expressed in Escherichia coli transformed CHQ and 2,6-dichlorohydroquinone to MA and 2-chloromaleylacetate, respectively. Our previous and present results indicate that UT26 (i) has two gene clusters for degradation of chlorinated aromatic compounds via hydroquinone-type intermediates and (ii) uses at least parts of both clusters for -HCH utilization.

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