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Journal of Bacteriology, February 2005, p. 854-861, Vol. 187, No. 3
0021-9193/05/$08.00+0     doi:10.1128/JB.187.3.854-861.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Characterization of a Truncated Lipoarabinomannan from the Actinomycete Turicella otitidis

Martine Gilleron,^1, Natalie J. Garton,^2,, Jérôme Nigou,¹ Thérèse Brando,¹ Germain Puzo,¹ and Iain C. Sutcliffe^2*

Institut de Pharmacologie et de Biologie Structurale du Centre National de la Recherche Scientifique, Toulouse, France,¹ Institute of Pharmacy, Chemistry and Biomedical Sciences, The University of Sunderland, Sunderland, United Kingdom²

Received 1 September 2004/ Accepted 22 October 2004

Lipoarabinomannan (LAM) lipoglycans have been characterized from a range of mycolic acid-containing actinomycetes and from the amycolate actinomycete Amycolatopsis sulphurea. To further understand the structural diversity of this family, we have characterized the lipoglycan of the otic commensal Turicella otitidis. T. otitidis LAM (TotLAM) has been determined to consist of a mannosyl phosphatidylinositol anchor unit carrying an ( 16)-linked mannan core and substituted with terminal-arabinosyl branches. Thus, TotLAM has a novel truncated LAM structure. Using the human monocytic THP-1 cell line, it was found that TotLAM exhibited only minimal ability to induce tumor necrosis factor alpha. These findings contribute further to our understanding of actinomycete LAM diversity and allow further speculation as to the correlation between LAM structure and the immunomodulatory activities of these lipoglycans.

M.G. and N.J.G. contributed equally to this study.

Present address: Department of Infection, Immunity and Inflammation, Leicester University, Leicester LE1 9HN, United Kingdom.

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