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Journal of Bacteriology, February 2005, p. 912-922, Vol. 187, No. 3
0021-9193/05/$08.00+0     doi:10.1128/JB.187.3.912-922.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Transcriptional Regulation of sitABCD of Salmonella enterica Serovar Typhimurium by MntR and Fur

Jack S. Ikeda,1,{dagger} Anuradha Janakiraman,1,{dagger},{ddagger} David G. Kehres,2 Michael E. Maguire,2 and James M. Slauch1,3*

Department of Microbiology,1 College of Medicine, University of Illinois at Urbana—Champaign, Urbana, Illinois ,3 Department of Pharmacology, Case Western Reserve University, Cleveland, Ohio2

Received 3 September 2004/ Accepted 1 November 2004

Salmonella enterica serovar Typhimurium has two manganese transport systems, MntH and SitABCD. MntH is a bacterial homolog of the eukaryotic natural resistance-associated macrophage protein 1 (Nramp1), and SitABCD is an ABC-type transporter. Previously we showed that mntH is negatively controlled at the transcriptional level by the trans-acting regulatory factors, MntR and Fur. In this study, we examined the transcriptional regulation of sitABCD and compared it to the transcriptional regulation of mntH by constructing lacZ fusions to the promoter regions with and without mutations in putative MntR and/or Fur binding sites. The presence of Mn caused transcriptional repression of the sitABCD and mntH promoters primarily via MntR, but Fur was also capable of some repression in response to Mn. Likewise, Fe in the medium repressed transcription of both sit and mntH primarily via Fur, although MntR was also involved in this response. Transcriptional control by MntR and Fur was disrupted by site-specific mutations in the putative MntR and Fur binding sites, respectively. Transcription of the sit operon was also affected by the oxygen level and growth phase, but the increased expression observed under high oxygen conditions and higher cell densities is consistent with decreased availability of metals required for repression by the metalloregulatory proteins.

* Corresponding author. Mailing address: Department of Microbiology, University of Illinois, B103 CLSL, 601 S. Goodwin Ave., Urbana, IL 61801. Phone: (217) 244-1956. Fax: (217) 244-6697. E-mail: slauch{at}uiuc.edu.

{dagger} J.S.I. and A.J. contributed equally to this study.

{ddagger} Present address: Department of Microbiology and Molecular Genetics, Harvard Medical School, Cambridge, MA 02139.

Journal of Bacteriology, February 2005, p. 912-922, Vol. 187, No. 3
0021-9193/05/$08.00+0     doi:10.1128/JB.187.3.912-922.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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