This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhang, Z.
Right arrow Articles by Saier, M. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhang, Z.
Right arrow Articles by Saier, M. H., Jr.

 Previous Article  |  Next Article 

Journal of Bacteriology, February 2005, p. 980-990, Vol. 187, No. 3
0021-9193/05/$08.00+0     doi:10.1128/JB.187.3.980-990.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Functional Interactions between the Carbon and Iron Utilization Regulators, Crp and Fur, in Escherichia coli

Zhongge Zhang,1 Guillermo Gosset,1,2 Ravi Barabote,1 Claudio S. Gonzalez,1 William A. Cuevas,3 and Milton H. Saier Jr.1*

Division of Biological Sciences, University of California at San Diego, La Jolla,1 Genencor International, Inc., Palo Alto, California,3 Departamento de Ingenieria Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, México2

Received 26 July 2004/ Accepted 26 October 2004

In Escherichia coli, the ferric uptake regulator (Fur) controls expression of the iron regulon in response to iron availability while the cyclic AMP receptor protein (Crp) regulates expression of the carbon regulon in response to carbon availability. We here identify genes subject to significant changes in expression level in response to the loss of both Fur and Crp. Many iron transport genes and several carbon metabolic genes are subject to dual control, being repressed by the loss of Crp and activated by the loss of Fur. However, the sodB gene, encoding superoxide dismutase, and the aceBAK operon, encoding the glyoxalate shunt enzymes, show the opposite responses, being activated by the loss of Crp and repressed by the loss of Fur. Several other genes including the sdhA-D, sucA-D, and fumA genes, encoding key constituents of the Krebs cycle, proved to be repressed by the loss of both transcription factors. Finally, the loss of both Crp and Fur activated a heterogeneous group of genes under {sigma}S control encoding, for example, the cyclopropane fatty acid synthase, Cfa, the glycogen synthesis protein, GlgS, the 30S ribosomal protein, S22, and the mechanosensitive channel protein, YggB. Many genes appeared to be regulated by the two transcription factors in an apparently additive fashion, but apparent positive or negative cooperativity characterized several putative Crp/Fur interactions. Relevant published data were evaluated, putative Crp and Fur binding sites were identified, and representative results were confirmed by real-time PCR. Molecular explanations for some, but not all, of these effects are provided.

* Corresponding author. Mailing address: Division of Biological Sciences, University of California at San Diego, La Jolla, CA 92093-0116. Phone: (858) 534-4084. Fax: (858) 534-7108. E-mail: msaier{at}ucsd.edu.

Journal of Bacteriology, February 2005, p. 980-990, Vol. 187, No. 3
0021-9193/05/$08.00+0     doi:10.1128/JB.187.3.980-990.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • De Lay, N., Gottesman, S. (2009). The Crp-Activated Small Noncoding Regulatory RNA CyaR (RyeE) Links Nutritional Status to Group Behavior. J. Bacteriol. 191: 461-476 [Abstract] [Full Text]  
  • Guillemet, M. L., Moreau, P. L. (2008). Fur-Dependent Detoxification of Organic Acids by rpoS Mutants during Prolonged Incubation under Aerobic, Phosphate Starvation Conditions. J. Bacteriol. 190: 5567-5575 [Abstract] [Full Text]  
  • Abed, N., Bickle, M., Mari, B., Schapira, M., Sanjuan-Espana, R., Robbe Sermesant, K., Moncorge, O., Mouradian-Garcia, S., Barbry, P., Rudkin, B. B., Fauvarque, M.-O., Michaud-Soret, I., Colas, P. (2007). A Comparative Analysis of Perturbations Caused by a Gene Knock-out, a Dominant Negative Allele, and a Set of Peptide Aptamers. Mol. Cell. Proteomics 6: 2110-2121 [Abstract] [Full Text]  
  • Liu, S., Hu, X., Lohrke, S. M., Baker, C. J., Buyer, J. S., de Souza, J. T., Roberts, D. P. (2007). Role of sdhA and pfkA and catabolism of reduced carbon during colonization of cucumber roots by Enterobacter cloacae. Microbiology 153: 3196-3209 [Abstract] [Full Text]  
  • Lorca, G. L., Ezersky, A., Lunin, V. V., Walker, J. R., Altamentova, S., Evdokimova, E., Vedadi, M., Bochkarev, A., Savchenko, A. (2007). Glyoxylate and Pyruvate Are Antagonistic Effectors of the Escherichia coli IclR Transcriptional Regulator. J. Biol. Chem. 282: 16476-16491 [Abstract] [Full Text]  
  • Lopez-Gomollon, S., Hernandez, J. A., Wolk, C. P., Peleato, M. L., Fillat, M. F. (2007). Expression of furA is modulated by NtcA and strongly enhanced in heterocysts of Anabaena sp. PCC 7120. Microbiology 153: 42-50 [Abstract] [Full Text]  
  • Pelosi, L., Kuhn, L., Guetta, D., Garin, J., Geiselmann, J., Lenski, R. E., Schneider, D. (2006). Parallel Changes in Global Protein Profiles During Long-Term Experimental Evolution in Escherichia coli. Genetics 173: 1851-1869 [Abstract] [Full Text]  
  • Cheng, Y., Li, J.-H., Shi, L., Wang, L., Latifi, A., Zhang, C.-C. (2006). A Pair of Iron-Responsive Genes Encoding Protein Kinases with a Ser/Thr Kinase Domain and a His Kinase Domain Are Regulated by NtcA in the Cyanobacterium Anabaena sp. Strain PCC 7120. J. Bacteriol. 188: 4822-4829 [Abstract] [Full Text]  
  • Leveille, S., Caza, M., Johnson, J. R., Clabots, C., Sabri, M., Dozois, C. M. (2006). Iha from an Escherichia coli Urinary Tract Infection Outbreak Clonal Group A Strain Is Expressed In Vivo in the Mouse Urinary Tract and Functions as a Catecholate Siderophore Receptor.. Infect. Immun. 74: 3427-3436 [Abstract] [Full Text]  
  • Munoz-Rojas, J., Bernal, P., Duque, E., Godoy, P., Segura, A., Ramos, J.-L. (2006). Involvement of Cyclopropane Fatty Acids in the Response of Pseudomonas putida KT2440 to Freeze-Drying. Appl. Environ. Microbiol. 72: 472-477 [Abstract] [Full Text]  
  • Grainger, D. C., Hurd, D., Harrison, M., Holdstock, J., Busby, S. J. W. (2005). Studies of the distribution of Escherichia coli cAMP-receptor protein and RNA polymerase along the E. coli chromosome. Proc. Natl. Acad. Sci. USA 102: 17693-17698 [Abstract] [Full Text]  
  • Mey, A. R., Wyckoff, E. E., Kanukurthy, V., Fisher, C. R., Payne, S. M. (2005). Iron and Fur Regulation in Vibrio cholerae and the Role of Fur in Virulence. Infect. Immun. 73: 8167-8178 [Abstract] [Full Text]  
  • Lorca, G. L., Chung, Y. J., Barabote, R. D., Weyler, W., Schilling, C. H., Saier, M. H. Jr (2005). Catabolite Repression and Activation in Bacillus subtilis: Dependency on CcpA, HPr, and HprK. J. Bacteriol. 187: 7826-7839 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»