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Journal of Bacteriology, February 2005, p. 1238-1245, Vol. 187, No. 4
0021-9193/05/$08.00+0     doi:10.1128/JB.187.4.1238-1245.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

A Protein Secretion Pathway Critical for Mycobacterium tuberculosis Virulence Is Conserved and Functional in Mycobacterium smegmatis

Scott E. Converse and Jeffery S. Cox*

Department of Microbiology and Immunology, University of California, San Francisco, California

Received 22 July 2004/ Accepted 8 November 2004

The Snm protein secretion system is a critical determinant of Mycobacterium tuberculosis virulence. However, genes encoding components of this pathway are conserved among all mycobacteria, including the nonpathogenic saprophyte Mycobacterium smegmatis. We show that the Snm system is operational in M. smegmatis and that secretion of its homologous ESAT-6 and CFP-10 substrates is regulated by growth conditions. Importantly, we show that Snm secretion in M. smegmatis requires genes that are homologous to those required for secretion in M. tuberculosis. Using a gene knockout strategy in M. smegmatis, we have also discovered four new gene products that are essential for Snm secretion, including the serine protease mycosin 1. Despite the evolutionary distance between M. smegmatis and M. tuberculosis, the M. smegmatis Snm system can secrete the M. tuberculosis ESAT-6 and CFP-10 proteins, suggesting that substrate recognition is also conserved between the two species. M. smegmatis, therefore, represents a powerful system to study the multicomponent Snm secretory machine and to understand the role of this conserved system in mycobacterial biology.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of California, San Francisco, MBGH N312A, San Francisco, CA 94143-2200. Phone: (415) 502-4240. Fax: (415) 502-4315. E-mail: jcox{at}itsa.ucsf.edu.

Journal of Bacteriology, February 2005, p. 1238-1245, Vol. 187, No. 4
0021-9193/05/$08.00+0     doi:10.1128/JB.187.4.1238-1245.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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