Journal of Bacteriology, February 2005, p. 1511-1514, Vol. 187, No. 4
0021-9193/05/$08.00+0 doi:10.1128/JB.187.4.1511-1514.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.
Alpha-Subunit Positions Methionine 180 and Glutamate 214 of Pseudomonas stutzeri OX1 Toluene-o-Xylene Monooxygenase Influence Catalysis
Gönül Vardar and
Thomas K. Wood*
Departments of Chemical Engineering and Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut
Received 3 November 2004/
Accepted 17 November 2004
Alpha-subunit position M180 of toluene-o-xylene monooxygenase influences the regiospecific oxidation of aromatics (e.g., from o-cresol, M180H forms 3-methylcatechol, methylhydroquinone, and 4-methylresorcinol, whereas the wild type forms only 3-methylcatechol). Position E214 influences the rate of reaction (e.g., E214G increases p-nitrophenol oxidation 15-fold) by controlling substrate entrance and product efflux as a gate residue.
* Corresponding author. Mailing address: Department of Chemical Engineering, University of Connecticut, Storrs, CT 06269-3222. Phone: (860) 486-2483. Fax: (860) 486-2959. E-mail: twood{at}engr.uconn.edu.
Journal of Bacteriology, February 2005, p. 1511-1514, Vol. 187, No. 4
0021-9193/05/$08.00+0 doi:10.1128/JB.187.4.1511-1514.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.
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Copyright © 2005 by the American Society for Microbiology. All rights reserved.