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Journal of Bacteriology, February 2005, p. 1511-1514, Vol. 187, No. 4
0021-9193/05/$08.00+0     doi:10.1128/JB.187.4.1511-1514.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Alpha-Subunit Positions Methionine 180 and Glutamate 214 of Pseudomonas stutzeri OX1 Toluene-o-Xylene Monooxygenase Influence Catalysis

Gönül Vardar and Thomas K. Wood*

Departments of Chemical Engineering and Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut

Received 3 November 2004/ Accepted 17 November 2004

Alpha-subunit position M180 of toluene-o-xylene monooxygenase influences the regiospecific oxidation of aromatics (e.g., from o-cresol, M180H forms 3-methylcatechol, methylhydroquinone, and 4-methylresorcinol, whereas the wild type forms only 3-methylcatechol). Position E214 influences the rate of reaction (e.g., E214G increases p-nitrophenol oxidation 15-fold) by controlling substrate entrance and product efflux as a gate residue.

* Corresponding author. Mailing address: Department of Chemical Engineering, University of Connecticut, Storrs, CT 06269-3222. Phone: (860) 486-2483. Fax: (860) 486-2959. E-mail: twood{at}engr.uconn.edu.

Journal of Bacteriology, February 2005, p. 1511-1514, Vol. 187, No. 4
0021-9193/05/$08.00+0     doi:10.1128/JB.187.4.1511-1514.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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  • Lalucat, J., Bennasar, A., Bosch, R., Garcia-Valdes, E., Palleroni, N. J. (2006). Biology of Pseudomonas stutzeri. Microbiol. Mol. Biol. Rev. 70: 510-547 [Abstract] [Full Text]  


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