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Journal of Bacteriology, March 2005, p. 1604-1611, Vol. 187, No. 5
0021-9193/05/$08.00+0     doi:10.1128/JB.187.5.1604-1611.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

The Metal Permease ZupT from Escherichia coli Is a Transporter with a Broad Substrate Spectrum

Gregor Grass,1 Sylvia Franke,2 Nadine Taudte,1 Dietrich H. Nies,1 Lisa M. Kucharski,3 Michael E. Maguire,3 and Christopher Rensing2*

Institut für Mikrobiologie, Martin-Luther-Universität, Halle, Germany,1 Department of Soil, Water, and Environmental Science, University of Arizona, Tucson, Arizona,2 Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio3

Received 28 October 2004/ Accepted 9 November 2004

The Escherichia coli zupT (formerly ygiE) gene encodes a cytoplasmic membrane protein (ZupT) related to members of the eukaryotic ZIP family of divalent metal ion transporters. Previously, ZupT was shown to be responsible for uptake of zinc. In this study, we show that ZupT is a divalent metal cation transporter of broad substrate specificity. An E. coli strain with a disruption in all known iron uptake systems could grow in the presence of chelators only if zupT was expressed. Heterologous expression of Arabidopsis thaliana ZIP1 could also alleviate iron deficiency in this E. coli strain, as could expression of indigenous mntH or feoABC. Transport studies with intact cells showed that ZupT facilitates uptake of 55Fe2+ similarly to uptake of MntH or Feo. Other divalent cations were also taken up by ZupT, as shown using 57Co2+. Expression of zupT rendered E. coli cells hypersensitive to Co2+ and sensitive to Mn2+. ZupT did not appear to be metal regulated: expression of a {Phi}(zupT-lacZ) operon fusion indicated that zupT is expressed constitutively at a low level.

* Corresponding author. Mailing address: Department of Soil, Water, and Environmental Science, University of Arizona, Shantz Bldg. #38, Rm. 424, Tucson, AZ 85721. Phone: (520) 626-8482. Fax: (520) 621-1647. E-mail: rensingc{at}ag.arizona.edu.

Journal of Bacteriology, March 2005, p. 1604-1611, Vol. 187, No. 5
0021-9193/05/$08.00+0     doi:10.1128/JB.187.5.1604-1611.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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