This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Grass, G. Articles by Rensing, C. Search for Related Content PubMed PubMed Citation Articles by Grass, G. Articles by Rensing, C.

The Metal Permease ZupT from Escherichia coli Is a Transporter with a Broad Substrate Spectrum

Institut für Mikrobiologie, Martin-Luther-Universität, Halle, Germany,¹ Department of Soil, Water, and Environmental Science, University of Arizona, Tucson, Arizona,² Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio³

Received 28 October 2004/ Accepted 9 November 2004

The Escherichia coli zupT (formerly ygiE) gene encodes a cytoplasmic membrane protein (ZupT) related to members of the eukaryotic ZIP family of divalent metal ion transporters. Previously, ZupT was shown to be responsible for uptake of zinc. In this study, we show that ZupT is a divalent metal cation transporter of broad substrate specificity. An E. coli strain with a disruption in all known iron uptake systems could grow in the presence of chelators only if zupT was expressed. Heterologous expression of Arabidopsis thaliana ZIP1 could also alleviate iron deficiency in this E. coli strain, as could expression of indigenous mntH or feoABC. Transport studies with intact cells showed that ZupT facilitates uptake of ⁵⁵Fe²⁺ similarly to uptake of MntH or Feo. Other divalent cations were also taken up by ZupT, as shown using ⁵⁷Co²⁺. Expression of zupT rendered E. coli cells hypersensitive to Co²⁺ and sensitive to Mn²⁺. ZupT did not appear to be metal regulated: expression of a (zupT-lacZ) operon fusion indicated that zupT is expressed constitutively at a low level.

This article has been cited by other articles:

• Helbig, K., Bleuel, C., Krauss, G. J., Nies, D. H. (2008). Glutathione and Transition-Metal Homeostasis in Escherichia coli. J. Bacteriol. 190: 5431-5438 [Abstract] [Full Text]  
• Courville, P., Urbankova, E., Rensing, C., Chaloupka, R., Quick, M., Cellier, M. F. M. (2008). Solute Carrier 11 Cation Symport Requires Distinct Residues in Transmembrane Helices 1 and 6. J. Biol. Chem. 283: 9651-9658 [Abstract] [Full Text]  
• Golding, G. R., Sparling, R., Kelly, C. A. (2008). Effect of pH on Intracellular Accumulation of Trace Concentrations of Hg(II) in Escherichia coli under Anaerobic Conditions, as Measured Using a mer-lux Bioreporter. Appl. Environ. Microbiol. 74: 667-675 [Abstract] [Full Text]  
• Thompson, K., Molina, R. M., Donaghey, T., Brain, J. D., Wessling-Resnick, M. (2007). Iron absorption by Belgrade rat pups during lactation. Am. J. Physiol. Gastrointest. Liver Physiol. 293: G640-G644 [Abstract] [Full Text]  
• Garcia, S. J., Gellein, K., Syversen, T., Aschner, M. (2007). Iron Deficient and Manganese Supplemented Diets Alter Metals and Transporters in the Developing Rat Brain. Toxicol Sci 95: 205-214 [Abstract] [Full Text]  
• Liuzzi, J. P., Aydemir, F., Nam, H., Knutson, M. D., Cousins, R. J. (2006). Zip14 (Slc39a14) mediates non-transferrin-bound iron uptake into cells. Proc. Natl. Acad. Sci. USA 103: 13612-13617 [Abstract] [Full Text]  
• Runyen-Janecky, L., Dazenski, E., Hawkins, S., Warner, L. (2006). Role and Regulation of the Shigella flexneri Sit and MntH Systems.. Infect. Immun. 74: 4666-4672 [Abstract] [Full Text]  
• Eshaghi, S., Niegowski, D., Kohl, A., Molina, D. M., Lesley, S. A., Nordlund, P. (2006). Crystal Structure of a Divalent Metal Ion Transporter CorA at 2.9 Angstrom Resolution.. Science 313: 354-357 [Abstract] [Full Text]  
• Haraszthy, V. I., Jordan, S. F., Zambon, J. J. (2006). Identification of Fur-regulated genes in Actinobacillus actinomycetemcomitans.. Microbiology 152: 787-796 [Abstract] [Full Text]  
• Bleuel, C., Grosse, C., Taudte, N., Scherer, J., Wesenberg, D., Krauss, G. J., Nies, D. H., Grass, G. (2005). TolC Is Involved in Enterobactin Efflux across the Outer Membrane of Escherichia coli. J. Bacteriol. 187: 6701-6707 [Abstract] [Full Text]