This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Klena, J. Articles by Chen, T. Search for Related Content PubMed PubMed Citation Articles by Klena, J. Articles by Chen, T.

 Previous Article  |  Next Article 

Journal of Bacteriology, March 2005, p. 1710-1715, Vol. 187, No. 5
0021-9193/05/$08.00+0     doi:10.1128/JB.187.5.1710-1715.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

The Core Lipopolysaccharide of Escherichia coli Is a Ligand for the Dendritic-Cell-Specific Intercellular Adhesion Molecule Nonintegrin CD209 Receptor

School of Molecular Biosciences, Washington State University, Pullman, Washington,¹ Department of Microbiology and Immunology, Division of Infectious Diseases, Walther Oncology Center, Indiana University School of Medicine, Indianapolis, Indiana,² Virus and Immunity Group, Department of Virology, Institut Pasteur, Paris, France,³ Department of Microbiology and Immunology, Baylor College of Medicine, Houston, Texas⁴

Received 1 November 2004/ Accepted 16 November 2004

The dendritic-cell-specific intercellular adhesion molecule nonintegrin (DC-SIGN) CD209 is a receptor for Escherichia coli K-12 that promotes bacterial adherence and phagocytosis. However, the ligand of E. coli for DC-SIGN has not yet been identified. In this study, we found that DC-SIGN did not mediate the phagocytosis of several pathogenic strains of E. coli, including enteropathogenic E. coli, enterohemorrhagic E. coli, enterotoxigenic E. coli, and uropathogenic E. coli, in dendritic cells or HeLa cells expressing human DC-SIGN antigen. However, we showed that an outer core lipopolysaccharide (LPS) (rough) mutant, unlike an inner core LPS (deep rough) mutant or O-antigen-expressing recombinant of E. coli K-12 was phagocytosed. These results demonstrate that the host cells expressing DC-SIGN can phagocytose E. coli in part by interacting with the complete core region of the LPS molecule. These results provide a mechanism for how O antigen acts as an antiphagocytic factor.

This article has been cited by other articles:

• Zhang, P., Skurnik, M., Zhang, S.-S., Schwartz, O., Kalyanasundaram, R., Bulgheresi, S., He, J. J., Klena, J. D., Hinnebusch, B. J., Chen, T. (2008). Human Dendritic Cell-Specific Intercellular Adhesion Molecule-Grabbing Nonintegrin (CD209) Is a Receptor for Yersinia pestis That Promotes Phagocytosis by Dendritic Cells. Infect. Immun. 76: 2070-2079 [Abstract] [Full Text]  
• Jauffred, L., Callisen, T. H., Oddershede, L. B. (2007). Visco-Elastic Membrane Tethers Extracted from Escherichia coli by Optical Tweezers. Biophys. J 93: 4068-4075 [Abstract] [Full Text]  
• Zhang, P., Snyder, S., Feng, P., Azadi, P., Zhang, S., Bulgheresi, S., Sanderson, K. E., He, J., Klena, J., Chen, T. (2006). Role of N-Acetylglucosamine within Core Lipopolysaccharide of Several Species of Gram-Negative Bacteria in Targeting the DC-SIGN (CD209). J. Immunol. 177: 4002-4011 [Abstract] [Full Text]  
• Caminschi, I., Corbett, A. J, Zahra, C., Lahoud, M., Lucas, K. M, Sofi, M., Vremec, D., Gramberg, T., Pohlmann, S., Curtis, J., Handman, E., van Dommelen, S. L H, Fleming, P., Degli-Esposti, M. A, Shortman, K., Wright, M. D (2006). Functional comparison of mouse CIRE/mouse DC-SIGN and human DC-SIGN. Int Immunol 18: 741-753 [Abstract] [Full Text]  
• Bulgheresi, S., Schabussova, I., Chen, T., Mullin, N. P., Maizels, R. M., Ott, J. A. (2006). A New C-Type Lectin Similar to the Human Immunoreceptor DC-SIGN Mediates Symbiont Acquisition by a Marine Nematode. Appl. Environ. Microbiol. 72: 2950-2956 [Abstract] [Full Text]  
• Murray, G. L., Attridge, S. R., Morona, R. (2006). Altering the Length of the Lipopolysaccharide O Antigen Has an Impact on the Interaction of Salmonella enterica Serovar Typhimurium with Macrophages and Complement.. J. Bacteriol. 188: 2735-2739 [Abstract] [Full Text]  
• Zhang, P., Schwartz, O., Pantelic, M., Li, G., Knazze, Q., Nobile, C., Radovich, M., He, J., Hong, S.-C., Klena, J., Chen, T. (2006). DC-SIGN (CD209) recognition of Neisseria gonorrhoeae is circumvented by lipooligosaccharide variation. J. Leukoc. Biol. 79: 731-738 [Abstract] [Full Text]  
• Nagaoka, K., Takahara, K., Tanaka, K., Yoshida, H., Steinman, R. M., Saitoh, S.-i., Akashi-Takamura, S., Miyake, K., Kang, Y. S., Park, C. G., Inaba, K. (2005). Association of SIGNR1 with TLR4-MD-2 enhances signal transduction by recognition of LPS in gram-negative bacteria. Int Immunol 17: 827-836 [Abstract] [Full Text]