This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rasmussen, T. B.
Right arrow Articles by Givskov, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rasmussen, T. B.
Right arrow Articles by Givskov, M.

 Previous Article  |  Next Article 

Journal of Bacteriology, March 2005, p. 1799-1814, Vol. 187, No. 5
0021-9193/05/$08.00+0     doi:10.1128/JB.187.5.1799-1814.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Screening for Quorum-Sensing Inhibitors (QSI) by Use of a Novel Genetic System, the QSI Selector

Thomas Bovbjerg Rasmussen,1 Thomas Bjarnsholt,1 Mette Elena Skindersoe,1 Morten Hentzer,2 Peter Kristoffersen,1 Manuela Köte,3 John Nielsen,4 Leo Eberl,3,5 and Michael Givskov1*

Center for Biomedical Microbiology, BioCentrum-DTU, Technical University of Denmark, Kgs. Lyngby,1 Carlsberg Research Center, Biosector, Valby,2 Department of Chemistry, Royal Veterinary and Agricultural University of Denmark, Frederiksberg, Denmark,4 Lehrstuhl für Mikrobiologie, Technische Universitat München, Freising, Germany,3 Department of Microbiology, University of Zürich, Zürich, Switzerland5

Received 10 April 2004/ Accepted 22 October 2004

With the widespread appearance of antibiotic-resistant bacteria, there is an increasing demand for novel strategies to control infectious diseases. Furthermore, it has become apparent that the bacterial life style also contributes significantly to this problem. Bacteria living in the biofilm mode of growth tolerate conventional antimicrobial treatments. The discovery that many bacteria use quorum-sensing (QS) systems to coordinate virulence and biofilm development has pointed out a new, promising target for antimicrobial drugs. We constructed a collection of screening systems, QS inhibitor (QSI) selectors, which enabled us to identify a number of novel QSIs among natural and synthetic compound libraries. The two most active were garlic extract and 4-nitro-pyridine-N-oxide (4-NPO). GeneChip-based transcriptome analysis revealed that garlic extract and 4-NPO had specificity for QS-controlled virulence genes in Pseudomonas aeruginosa. These two QSIs also significantly reduced P. aeruginosa biofilm tolerance to tobramycin treatment as well as virulence in a Caenorhabditis elegans pathogenesis model.

* Corresponding author. Mailing address: Center for Biomedical Microbiology, BioCentrum-DTU, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark. Phone: 45 4525 2769. Fax: 45 4588 7328. E-mail: immg{at}pop.dtu.dk.

Journal of Bacteriology, March 2005, p. 1799-1814, Vol. 187, No. 5
0021-9193/05/$08.00+0     doi:10.1128/JB.187.5.1799-1814.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Nahum, L. A., Goswami, S., Serres, M. H. (2009). Protein families reflect the metabolic diversity of organisms and provide support for functional prediction. Physiol. Genomics 38: 250-260 [Abstract] [Full Text]  
  • Zhang, M., Jiao, X.-d., Hu, Y.-h., Sun, L. (2009). Attenuation of Edwardsiella tarda Virulence by Small Peptides That Interfere with LuxS/Autoinducer Type 2 Quorum Sensing. Appl. Environ. Microbiol. 75: 3882-3890 [Abstract] [Full Text]  
  • Yang, L., Rybtke, M. T., Jakobsen, T. H., Hentzer, M., Bjarnsholt, T., Givskov, M., Tolker-Nielsen, T. (2009). Computer-Aided Identification of Recognized Drugs as Pseudomonas aeruginosa Quorum-Sensing Inhibitors. Antimicrob. Agents Chemother. 53: 2432-2443 [Abstract] [Full Text]  
  • Shepherd, R. W., Lindow, S. E. (2009). Two Dissimilar N-Acyl-Homoserine Lactone Acylases of Pseudomonas syringae Influence Colony and Biofilm Morphology. Appl. Environ. Microbiol. 75: 45-53 [Abstract] [Full Text]  
  • Skindersoe, M. E., Alhede, M., Phipps, R., Yang, L., Jensen, P. O., Rasmussen, T. B., Bjarnsholt, T., Tolker-Nielsen, T., Hoiby, N., Givskov, M. (2008). Effects of Antibiotics on Quorum Sensing in Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 52: 3648-3663 [Abstract] [Full Text]  
  • Overhage, J., Campisano, A., Bains, M., Torfs, E. C. W., Rehm, B. H. A., Hancock, R. E. W. (2008). Human Host Defense Peptide LL-37 Prevents Bacterial Biofilm Formation. Infect. Immun. 76: 4176-4182 [Abstract] [Full Text]  
  • Popat, R., Crusz, S. A., Diggle, S. P. (2008). The social behaviours of bacterial pathogens. Br Med Bull 87: 63-75 [Abstract] [Full Text]  
  • Willcox, M. D. P., Zhu, H., Conibear, T. C. R., Hume, E. B. H., Givskov, M., Kjelleberg, S., Rice, S. A. (2008). Role of quorum sensing by Pseudomonas aeruginosa in microbial keratitis and cystic fibrosis. Microbiology 154: 2184-2194 [Abstract] [Full Text]  
  • Adonizio, A., Leal, S. M. Jr, Ausubel, F. M., Mathee, K. (2008). Attenuation of Pseudomonas aeruginosa virulence by medicinal plants in a Caenorhabditis elegans model system. J Med Microbiol 57: 809-813 [Abstract] [Full Text]  
  • von Bodman, S. B., Willey, J. M., Diggle, S. P. (2008). Cell-Cell Communication in Bacteria: United We Stand. J. Bacteriol. 190: 4377-4391 [Full Text]  
  • Adonizio, A., Kong, K.-F., Mathee, K. (2008). Inhibition of Quorum Sensing-Controlled Virulence Factor Production in Pseudomonas aeruginosa by South Florida Plant Extracts. Antimicrob. Agents Chemother. 52: 198-203 [Abstract] [Full Text]  
  • Sanchez-Contreras, M., Bauer, W. D, Gao, M., Robinson, J. B, Allan Downie, J (2007). Quorum-sensing regulation in rhizobia and its role in symbiotic interactions with legumes. Phil Trans R Soc B 362: 1149-1163 [Abstract] [Full Text]  
  • Bjarnsholt, T., Givskov, M. (2007). Quorum-sensing blockade as a strategy for enhancing host defences against bacterial pathogens. Phil Trans R Soc B 362: 1213-1222 [Abstract] [Full Text]  
  • Christensen, L. D., Moser, C., Jensen, P. O., Rasmussen, T. B., Christophersen, L., Kjelleberg, S., Kumar, N., Hoiby, N., Givskov, M., Bjarnsholt, T. (2007). Impact of Pseudomonas aeruginosa quorum sensing on biofilm persistence in an in vivo intraperitoneal foreign-body infection model. Microbiology 153: 2312-2320 [Abstract] [Full Text]  
  • Taylor, M. W., Radax, R., Steger, D., Wagner, M. (2007). Sponge-Associated Microorganisms: Evolution, Ecology, and Biotechnological Potential. Microbiol. Mol. Biol. Rev. 71: 295-347 [Abstract] [Full Text]  
  • Bottomley, M. J., Muraglia, E., Bazzo, R., Carfi, A. (2007). Molecular Insights into Quorum Sensing in the Human Pathogen Pseudomonas aeruginosa from the Structure of the Virulence Regulator LasR Bound to Its Autoinducer. J. Biol. Chem. 282: 13592-13600 [Abstract] [Full Text]  
  • Gonzalez, J. E., Keshavan, N. D. (2006). Messing with Bacterial Quorum Sensing. Microbiol. Mol. Biol. Rev. 70: 859-875 [Abstract] [Full Text]  
  • Karamanoli, K., Lindow, S. E. (2006). Disruption of N-Acyl Homoserine Lactone-Mediated Cell Signaling and Iron Acquisition in Epiphytic Bacteria by Leaf Surface Compounds. Appl. Environ. Microbiol. 72: 7678-7686 [Abstract] [Full Text]  
  • Muh, U., Schuster, M., Heim, R., Singh, A., Olson, E. R., Greenberg, E. P. (2006). Novel Pseudomonas aeruginosa Quorum-Sensing Inhibitors Identified in an Ultra-High-Throughput Screen. Antimicrob. Agents Chemother. 50: 3674-3679 [Abstract] [Full Text]  
  • Koutsoudis, M. D., Tsaltas, D., Minogue, T. D., von Bodman, S. B. (2006). Quorum-sensing regulation governs bacterial adhesion, biofilm development, and host colonization in Pantoea stewartii subspecies stewartii. Proc. Natl. Acad. Sci. USA 103: 5983-5988 [Abstract] [Full Text]  
  • Rasmussen, T. B., Givskov, M. (2006). Quorum sensing inhibitors: a bargain of effects.. Microbiology 152: 895-904 [Abstract] [Full Text]  
  • Keshavan, N. D., Chowdhary, P. K., Haines, D. C., Gonzalez, J. E. (2005). L-Canavanine Made by Medicago sativa Interferes with Quorum Sensing in Sinorhizobium meliloti. J. Bacteriol. 187: 8427-8436 [Abstract] [Full Text]  
  • Bjarnsholt, T., Jensen, P. O., Rasmussen, T. B., Christophersen, L., Calum, H., Hentzer, M., Hougen, H.-P., Rygaard, J., Moser, C., Eberl, L., Hoiby, N., Givskov, M. (2005). Garlic blocks quorum sensing and promotes rapid clearing of pulmonary Pseudomonas aeruginosa infections. Microbiology 151: 3873-3880 [Abstract] [Full Text]  
  • Rasmussen, T. B., Skindersoe, M. E., Bjarnsholt, T., Phipps, R. K., Christensen, K. B., Jensen, P. O., Andersen, J. B., Koch, B., Larsen, T. O., Hentzer, M., Eberl, L., Hoiby, N., Givskov, M. (2005). Identity and effects of quorum-sensing inhibitors produced by Penicillium species. Microbiology 151: 1325-1340 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»