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The Promoter Region Rather than Its Downstream Inverted Repeat Sequence Is Responsible for Low-Level Transcription of the Thermostable Direct Hemolysin-Related Hemolysin (trh) Gene of Vibrio parahaemolyticus

Graduate School of Medicine,¹ Center for Southeast Asian Studies, Kyoto University, Yoshida, Sakyo-ku, Kyoto, Japan²

Received 12 April 2004/ Accepted 28 November 2004

We determined the transcriptional start site of the thermostable direct hemolysin-related hemolysin gene (trh) of Vibrio parahaemolyticus by using a PCR-based method and identified the promoter. Mutagenic analysis indicated that the promoter-bearing region rather than its downstream inverted repeat sequence was responsible for the low-revel of trh transcription.