Journal of Bacteriology, March 2005, p. 1849-1855, Vol. 187, No. 5
0021-9193/05/$08.00+0 doi:10.1128/JB.187.5.1849-1855.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.
The Promoter Region Rather than Its Downstream Inverted Repeat Sequence Is Responsible for Low-Level Transcription of the Thermostable Direct Hemolysin-Related Hemolysin (trh) Gene of Vibrio parahaemolyticus
Yoshitsugu Nakaguchi1,2 and
Mitsuaki Nishibuchi2*
Graduate School of Medicine,1
Center for Southeast Asian Studies, Kyoto University, Yoshida, Sakyo-ku, Kyoto, Japan2
Received 12 April 2004/
Accepted 28 November 2004
We determined the transcriptional start site of the thermostable direct hemolysin-related hemolysin gene (trh) of Vibrio parahaemolyticus by using a PCR-based method and identified the promoter. Mutagenic analysis indicated that the promoter-bearing region rather than its downstream inverted repeat sequence was responsible for the low-revel of trh transcription.
* Corresponding author. Mailing address: Center for Southeast Asian Studies, Kyoto University, 46 Shimoadachi-cho, Yoshida, Sakyo-ku, Kyoto 606-8501, Japan. Phone: 81-75-753-7367. Fax: 81-75-753-7350. E-mail: nisibuti{at}cseas.kyoto-u.ac.jp.
Journal of Bacteriology, March 2005, p. 1849-1855, Vol. 187, No. 5
0021-9193/05/$08.00+0 doi:10.1128/JB.187.5.1849-1855.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.
Copyright © 2005 by the American Society for Microbiology. All rights reserved.