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Involvement of P1 Adhesin in Gliding Motility of Mycoplasma pneumoniae as Revealed by the Inhibitory Effects of Antibody under Optimized Gliding Conditions

Shintaro Seto,^1, Tsuyoshi Kenri,² Tetsuo Tomiyama,³ and Makoto Miyata^1,4*

Department of Biology, Graduate School of Science, Osaka City University,¹ PRESTO, JST, Sumiyoshi-ku, Osaka,⁴ Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Musashimurayama,² Tomiyama Laboratories, Nerima-ku, Tokyo, Japan³

Received 15 September 2004/ Accepted 30 November 2004

To examine the participation of P1 adhesin in gliding of Mycoplasma pneumoniae, we examined the effects of an anti-P1 monoclonal antibody on individual gliding mycoplasmas. The antibody reduced the gliding speed and removed the gliding cells from the glass over time in a concentration-dependent manner but had only a slight effect on nongliding cells, suggesting that the conformational changes of P1 adhesin and its displacement are involved in the gliding mechanism.

Present address: Department of Oral Microbiology, Meikai University School of Dentistry, Keyakidai, Sakado, Saitama 350-0283, Japan.

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