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Journal of Bacteriology, March 2005, p. 2066-2076, Vol. 187, No. 6
0021-9193/05/$08.00+0     doi:10.1128/JB.187.6.2066-2076.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Cyclic AMP (cAMP) and cAMP Receptor Protein Influence both Synthesis and Uptake of Extracellular Autoinducer 2 in Escherichia coli

Liang Wang,1,2 Yoshifumi Hashimoto,1 Chen-Yu Tsao,1,3 James J. Valdes,4 and William E. Bentley1,3*

Center for Biosystems Research, University of Maryland Biotechnology Institute,1 Department of Cell Biology and Molecular Genetics,2 Department of Chemical Engineering, University of Maryland, College Park,3 U.S. Army Edgewood Chemical Biological Center, Aberdeen Proving Ground, Maryland4

Received 9 October 2004/ Accepted 6 December 2004

Bacterial autoinducer 2 (AI-2) is proposed to be an interspecies mediator of cell-cell communication that enables cells to operate at the multicellular level. Many environmental stimuli have been shown to affect the extracellular AI-2 levels, carbon sources being among the most important. In this report, we show that both AI-2 synthesis and uptake in Escherichia coli are subject to catabolite repression through the cyclic AMP (cAMP)-CRP complex, which directly stimulates transcription of the lsr (for "luxS regulated") operon and indirectly represses luxS expression. Specifically, cAMP-CRP is shown to bind to a CRP binding site located in the upstream region of the lsr promoter and works with the LsrR repressor to regulate AI-2 uptake. The functions of the lsr operon and its regulators, LsrR and LsrK, previously reported in Salmonella enterica serovar Typhimurium, are confirmed here for E. coli. The elucidation of cAMP-CRP involvement in E. coli autoinduction impacts many areas, including the growth of E. coli in fermentation processes.

* Corresponding author. Mailing address: Center for Biosystems Research, University of Maryland Biotechnology Institute, College Park, MD 20742. Phone: (301) 405-4321. Fax: (301) 314-9075. E-mail: bentley{at}eng.umd.edu.

Journal of Bacteriology, March 2005, p. 2066-2076, Vol. 187, No. 6
0021-9193/05/$08.00+0     doi:10.1128/JB.187.6.2066-2076.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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