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 Previous Article

Journal of Bacteriology, March 2005, p. 2218-2223, Vol. 187, No. 6
0021-9193/05/$08.00+0     doi:10.1128/JB.187.6.2218-2223.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Influence of Amino Acid Substitutions in the Leader Peptide on Maturation and Secretion of Mesentericin Y105 by Leuconostoc mesenteroides

Willy Aucher, Christian Lacombe, Arnaud Héquet, Jacques Frère, and Jean-Marc Berjeaud*

Institut de Biologie Moléculaire et d'Ingénierie Génétique, Equipe de Microbiologie Fondamentale et Appliquée, UMR CNRS 6008, Université de Poitiers, Poitiers, France

Received 27 September 2004/ Accepted 10 December 2004

By site-specific mutagenesis, the hydrophobic conserved amino acids and the C-terminal GG doublet of the leader peptide of pre-mesentericin Y105 were demonstrated to be critical for optimal secretion of mesentericin Y105, as well as for the maturation of the pre-bacteriocin by the protease portion of the ABC transporter MesD.

* Corresponding author. Mailing address: Microbiologie Fondamentale et Appliquée, IBMIG, 40 ave. du Recteur Pineau, 86022 Poitiers Cedex, France. Phone: (33) 5 49 45 40 06. Fax: (33) 5 49 45 35 03. E-mail: jean-marc.berjeaud{at}univ-poitiers.fr.

Journal of Bacteriology, March 2005, p. 2218-2223, Vol. 187, No. 6
0021-9193/05/$08.00+0     doi:10.1128/JB.187.6.2218-2223.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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