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Journal of Bacteriology, April 2005, p. 2257-2260, Vol. 187, No. 7
0021-9193/05/$08.00+0     doi:10.1128/JB.187.7.2257-2260.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Regulation of the Vibrio anguillarum Metalloprotease EmpA by Posttranslational Modification

Andrew M. Staroscik, Steven M. Denkin,{dagger} and David R. Nelson*

Department of Cell and Molecular Biology, University of Rhode Island, Kingston, Rhode Island

Received 31 August 2004/ Accepted 21 December 2004

The zinc metalloprotease EmpA is a virulence factor in the fish pathogen Vibrio anguillarum. Previous studies have shown that two strains of V. anguillarum regulate empA differently. Strain M93Sm exhibits protease activity only in the presence of fish gastrointestinal mucus, while protease activity is detected in NB10 culture supernatant under all stationary-phase conditions. In this study, we use real-time reverse transcription-PCR to show that even in conditions where no protease activity is detected, empA transcription occurs. Western blot analysis revealed that EmpA is secreted as a ~48-kDa proenzyme and that activation occurs extracellularly by the removal of a ~10-kDa peptide. The presence of stable extracellular pro-EmpA in M93Sm culture supernatants suggests that activation of EmpA is not autolytic.

* Corresponding author. Mailing address: Department of Cell and Molecular Biology, 117 Morrill Science Building, University of Rhode Island, Kingston, RI 02881. Phone: (401) 874-5902. Fax: (401) 874-2202. E-mail: dnelson{at}uri.edu.

{dagger} Present address: Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205.

Journal of Bacteriology, April 2005, p. 2257-2260, Vol. 187, No. 7
0021-9193/05/$08.00+0     doi:10.1128/JB.187.7.2257-2260.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Varina, M., Denkin, S. M., Staroscik, A. M., Nelson, D. R. (2008). Identification and Characterization of Epp, the Secreted Processing Protease for the Vibrio anguillarum EmpA Metalloprotease. J. Bacteriol. 190: 6589-6597 [Abstract] [Full Text]  


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