This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mohedano, M. L.
Right arrow Articles by Wells, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mohedano, M. L.
Right arrow Articles by Wells, J. M.

 Previous Article  |  Next Article 

Journal of Bacteriology, April 2005, p. 2357-2367, Vol. 187, No. 7
0021-9193/05/$08.00+0     doi:10.1128/JB.187.7.2357-2367.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Evidence that the Essential Response Regulator YycF in Streptococcus pneumoniae Modulates Expression of Fatty Acid Biosynthesis Genes and Alters Membrane Composition{dagger}

M. Luz Mohedano,1,{ddagger} Karin Overweg,2,{ddagger} Alicia de la Fuente,1 Mark Reuter,2 Silvia Altabe,3 Francis Mulholland,2 Diego de Mendoza,3 Paloma López,1* and Jerry M. Wells2

Departamento de Estructura y Función de Proteínas, Centro de Investigaciones Biológicas (CSIC), Madrid, Spain,1 Institute of Food Research, Norwich Research Park, Norwich, United Kingdom,2 Instituto de Biología Molecular y Celular de Rosario and Departamento de Microbiología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina3

Received 15 October 2004/ Accepted 14 December 2004

The YycFG two-component system, originally identified in Bacillus subtilis, is highly conserved among gram-positive bacteria with low G+C contents. In Streptococcus pneumoniae, the YycF response regulator has been reported to be essential for cell growth, but the signal to which it responds and the gene members of the regulon remain unclear. In order to investigate the role of YycFG in S. pneumoniae, we increased the expression of yycF by using a maltose-inducible vector and analyzed the genome-wide effects on transcription and protein expression during the course of yycF expression. The induction of yycF expression increased histidine kinase yycG transcript levels, suggesting an autoregulation of the yycFG operon. Evidence from both proteomic and microarray transcriptome studies as well as analyses of membrane fatty acid composition indicated that YycFG is involved in the regulation of fatty acid biosynthesis pathways and in determining fatty acid chain lengths in membrane lipids. In agreement with recent transcriptome data on pneumococcal cells depleted of YycFG, we also identified several other potential members of the YycFG regulon that are required for virulence and cell wall biosynthesis and metabolism.

* Corresponding author. Mailing address: Centro de Investigaciones Biológicas, Ramiro de Maeztu, 9, 28040 Madrid, Spain. Phone: (34) 918373112, ext. 4202. Fax: (34) 915360432. E-mail: plg{at}cib.csic.es.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

{ddagger} M.L.M. and K.O. contributed equally to this work.

Journal of Bacteriology, April 2005, p. 2357-2367, Vol. 187, No. 7
0021-9193/05/$08.00+0     doi:10.1128/JB.187.7.2357-2367.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Senadheera, D., Krastel, K., Mair, R., Persadmehr, A., Abranches, J., Burne, R. A., Cvitkovitch, D. G. (2009). Inactivation of VicK Affects Acid Production and Acid Survival of Streptococcus mutans. J. Bacteriol. 191: 6415-6424 [Abstract] [Full Text]  
  • Kosowska-Shick, K., Clark, C., Pankuch, G. A., McGhee, P., Dewasse, B., Beachel, L., Appelbaum, P. C. (2009). Activity of Telavancin against Staphylococci and Enterococci Determined by MIC and Resistance Selection Studies. Antimicrob. Agents Chemother. 53: 4217-4224 [Abstract] [Full Text]  
  • Tremblay, Y. D. N., Lo, H., Li, Y.-H., Halperin, S. A., Lee, S. F. (2009). Expression of the Streptococcus mutans essential two-component regulatory system VicRK is pH and growth-phase dependent and controlled by the LiaFSR three-component regulatory system. Microbiology 155: 2856-2865 [Abstract] [Full Text]  
  • Mishra, N. N., Yang, S.-J., Sawa, A., Rubio, A., Nast, C. C., Yeaman, M. R., Bayer, A. S. (2009). Analysis of Cell Membrane Characteristics of In Vitro-Selected Daptomycin-Resistant Strains of Methicillin-Resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 53: 2312-2318 [Abstract] [Full Text]  
  • Barendt, S. M., Land, A. D., Sham, L.-T., Ng, W.-L., Tsui, H.-C. T., Arnold, R. J., Winkler, M. E. (2009). Influences of Capsule on Cell Shape and Chain Formation of Wild-Type and pcsB Mutants of Serotype 2 Streptococcus pneumoniae. J. Bacteriol. 191: 3024-3040 [Abstract] [Full Text]  
  • Winkler, M. E., Hoch, J. A. (2008). Essentiality, Bypass, and Targeting of the YycFG (VicRK) Two-Component Regulatory System in Gram-Positive Bacteria. J. Bacteriol. 190: 2645-2648 [Full Text]  
  • Jones, T., Yeaman, M. R., Sakoulas, G., Yang, S.-J., Proctor, R. A., Sahl, H.-G., Schrenzel, J., Xiong, Y. Q., Bayer, A. S. (2008). Failures in Clinical Treatment of Staphylococcus aureus Infection with Daptomycin Are Associated with Alterations in Surface Charge, Membrane Phospholipid Asymmetry, and Drug Binding. Antimicrob. Agents Chemother. 52: 269-278 [Abstract] [Full Text]  
  • Dubrac, S., Boneca, I. G., Poupel, O., Msadek, T. (2007). New Insights into the WalK/WalR (YycG/YycF) Essential Signal Transduction Pathway Reveal a Major Role in Controlling Cell Wall Metabolism and Biofilm Formation in Staphylococcus aureus. J. Bacteriol. 189: 8257-8269 [Abstract] [Full Text]  
  • Chan, Y. C., Raengpradub, S., Boor, K. J., Wiedmann, M. (2007). Microarray-Based Characterization of the Listeria monocytogenes Cold Regulon in Log- and Stationary-Phase Cells. Appl. Environ. Microbiol. 73: 6484-6498 [Abstract] [Full Text]  
  • Ahn, S.-J., Burne, R. A. (2007). Effects of Oxygen on Biofilm Formation and the AtlA Autolysin of Streptococcus mutans. J. Bacteriol. 189: 6293-6302 [Abstract] [Full Text]  
  • Standish, A. J., Stroeher, U. H., Paton, J. C. (2007). The Pneumococcal Two-Component Signal Transduction System RR/HK06 Regulates CbpA and PspA by Two Distinct Mechanisms. J. Bacteriol. 189: 5591-5600 [Abstract] [Full Text]  
  • Deng, D.M., Liu, M.J., ten Cate, J.M., Crielaard, W. (2007). The VicRK System of Streptococcus mutans Responds to Oxidative Stress. JDR 86: 606-610 [Abstract] [Full Text]  
  • Wall, T., Bath, K., Britton, R. A., Jonsson, H., Versalovic, J., Roos, S. (2007). The Early Response to Acid Shock in Lactobacillus reuteri Involves the ClpL Chaperone and a Putative Cell Wall-Altering Esterase. Appl. Environ. Microbiol. 73: 3924-3935 [Abstract] [Full Text]  
  • Szurmant, H., Mohan, M. A., Imus, P. M., Hoch, J. A. (2007). YycH and YycI Interact To Regulate the Essential YycFG Two-Component System in Bacillus subtilis. J. Bacteriol. 189: 3280-3289 [Abstract] [Full Text]  
  • Ma, Z., Zhang, J.-R. (2007). RR06 Activates Transcription of spr1996 and cbpA in Streptococcus pneumoniae. J. Bacteriol. 189: 2497-2509 [Abstract] [Full Text]  
  • Fadda, D., Santona, A., D'Ulisse, V., Ghelardini, P., Ennas, M. G., Whalen, M. B., Massidda, O. (2007). Streptococcus pneumoniae DivIVA: Localization and Interactions in a MinCD-Free Context. J. Bacteriol. 189: 1288-1298 [Abstract] [Full Text]  
  • de Been, M., Francke, C., Moezelaar, R., Abee, T., Siezen, R. J. (2006). Comparative analysis of two-component signal transduction systems of Bacillus cereus, Bacillus thuringiensis and Bacillus anthracis.. Microbiology 152: 3035-3048 [Abstract] [Full Text]  
  • Lioy, V. S., Martin, M. T., Camacho, A. G., Lurz, R., Antelmann, H., Hecker, M., Hitchin, E., Ridge, Y., Wells, J. M., Alonso, J. C. (2006). pSM19035-encoded {zeta} toxin induces stasis followed by death in a subpopulation of cells.. Microbiology 152: 2365-2379 [Abstract] [Full Text]  
  • Liu, M., Hanks, T. S., Zhang, J., McClure, M. J., Siemsen, D. W., Elser, J. L., Quinn, M. T., Lei, B. (2006). Defects in ex vivo and in vivo growth and sensitivity to osmotic stress of group A Streptococcus caused by interruption of response regulator gene vicR.. Microbiology 152: 967-978 [Abstract] [Full Text]  
  • Paterson, G. K., Blue, C. E., Mitchell, T. J. (2006). Role of two-component systems in the virulence of Streptococcus pneumoniae.. J Med Microbiol 55: 355-363 [Abstract] [Full Text]  
  • Sun, J., Zheng, L., Landwehr, C., Yang, J., Ji, Y. (2005). Identification of a Novel Essential Two-Component Signal Transduction System, YhcSR, in Staphylococcus aureus. J. Bacteriol. 187: 7876-7880 [Abstract] [Full Text]  
  • Ng, W.-L., Tsui, H.-C. T., Winkler, M. E. (2005). Regulation of the pspA Virulence Factor and Essential pcsB Murein Biosynthetic Genes by the Phosphorylated VicR (YycF) Response Regulator in Streptococcus pneumoniae. J. Bacteriol. 187: 7444-7459 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»