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Journal of Bacteriology, April 2005, p. 2448-2457, Vol. 187, No. 7
0021-9193/05/$08.00+0     doi:10.1128/JB.187.7.2448-2457.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Inhibition of Salmonella enterica Serovar Typhimurium Lipopolysaccharide Deacylation by Aminoarabinose Membrane Modification

Kiyoshi Kawasaki,1,2 Robert K. Ernst,3 and Samuel I. Miller1,3,4*

Departments of Microbiology,1 Medicine,3 Genome Sciences, University of Washington, Seattle, Washington,4 Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Tokyo, Japan2

Received 27 September 2004/ Accepted 21 December 2004

Salmonella enterica serovar Typhimurium remodels the lipid A component of lipopolysaccharide, a major component of the outer membrane, to survive within animals. The activation of the sensor kinase PhoQ in host environments increases the synthesis of enzymes that deacylate, palmitoylate, hydroxylate, and attach aminoarabinose to lipid A, also known as endotoxin. These modifications promote bacterial resistance to antimicrobial peptides and reduce the host recognition of lipid A by Toll-like receptor 4. The Salmonella lipid A 3-O-deacylase, PagL, is an outer membrane protein whose expression is regulated by PhoQ. In S. enterica serovar Typhimurium strains that had the ability to add aminoarabinose to lipid A, 3-O-deacylated lipid A species were not detected, despite the PhoQ induction of PagL protein expression. In contrast, strains defective for the aminoarabinose modification of lipid A demonstrated in vivo PagL activity, indicating that this membrane modification inhibited PagL's enzymatic activity. Since not all lipid A molecules are modified with aminoarabinose upon PhoQ activation, these results cannot be ascribed to the substrate specificity of PagL. PagL-dependent deacylation was detected in sonically disrupted membranes and membranes treated with the nonionic detergent n-octyl-ß-D-glucopyranoside, suggesting that perturbation of the intact outer membrane releases PagL from posttranslational inhibition by aminoarabinose-containing membranes. Taken together, these results suggest that PagL enzymatic deacylation is posttranslationally inhibited by membrane environments, which either sequester PagL from its substrate or alter its conformation.

* Corresponding author. Mailing address: Departments of Medicine, Microbiology, and Genome Sciences, University of Washington, Health Sciences Building, K140, Box 357710, 1959 Pacific St. N.E., Seattle, WA 98195. Phone: (206) 616-5107. Fax: (206) 616-5109. E-mail: millersi{at}u.washington.edu.

Journal of Bacteriology, April 2005, p. 2448-2457, Vol. 187, No. 7
0021-9193/05/$08.00+0     doi:10.1128/JB.187.7.2448-2457.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Manabe, T., Kawasaki, K. (2008). Extracellular Loops of Lipid A 3-O-Deacylase PagL Are Involved in Recognition of Aminoarabinose-Based Membrane Modifications in Salmonella enterica Serovar Typhimurium. J. Bacteriol. 190: 5597-5606 [Abstract] [Full Text]  
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