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Journal of Bacteriology, April 2005, p. 2483-2490, Vol. 187, No. 7
0021-9193/05/$08.00+0     doi:10.1128/JB.187.7.2483-2490.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Crystal Structure of the Terminal Oxygenase Component of Cumene Dioxygenase from Pseudomonas fluorescens IP01{dagger}

Xuesong Dong,1 Shinya Fushinobu,1 Eriko Fukuda,1 Tohru Terada,1 Shugo Nakamura,1 Kentaro Shimizu,1 Hideaki Nojiri,2 Toshio Omori,3 Hirofumi Shoun,1 and Takayoshi Wakagi1*

Department of Biotechnology,1 Biotechnology Research Center, The University of Tokyo, Bunkyo-ku,2 Department of Industrial Chemistry, Shibaura Institute of Technology, Minato-ku, Tokyo, Japan3

Received 30 September 2004/ Accepted 10 December 2004

The crystal structure of the terminal component of the cumene dioxygenase multicomponent enzyme system of Pseudomonas fluorescens IP01 (CumDO) was determined at a resolution of 2.2 Å by means of molecular replacement by using the crystal structure of the terminal oxygenase component of naphthalene dioxygenase from Pseudomonas sp. strain NCIB 9816-4 (NphDO). The ligation of the two catalytic centers of CumDO (i.e., the nonheme iron and Rieske [2Fe-2S] centers) and the bridging between them in neighboring catalytic subunits by hydrogen bonds through a single amino acid residue, Asp231, are similar to those of NphDO. An unidentified external ligand, possibly dioxygen, was bound at the active site nonheme iron. The entrance to the active site of CumDO is different from the entrance to the active site of NphDO, as the two loops forming the lid exhibit great deviation. On the basis of the complex structure of NphDO, a biphenyl substrate was modeled in the substrate-binding pocket of CumDO. The residues surrounding the modeled biphenyl molecule include residues that have already been shown to be important for its substrate specificity by a number of engineering studies of biphenyl dioxygenases.

* Corresponding author. Mailing address: Department of Biotechnology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. Phone and fax: 81-3-5841-5152. E-mail: atwakag{at}mail.ecc.u-tokyo.ac.jp.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, April 2005, p. 2483-2490, Vol. 187, No. 7
0021-9193/05/$08.00+0     doi:10.1128/JB.187.7.2483-2490.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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