Journal of Bacteriology, April 2005, p. 2508-2512, Vol. 187, No. 7
0021-9193/05/$08.00+0 doi:10.1128/JB.187.7.2508-2512.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.
Novel Dephosphotetrahydromethanopterin Biosynthesis Genes Discovered via Mutagenesis in Methylobacterium extorquens AM1
Ludmila Chistoserdova,1*
Madeline E. Rasche,2 and
Mary E. Lidstrom1,3
Department of Chemical Engineering,1
Department of Microbiology, University of Washington, Seattle, Washington,3
Microbiology and Cell Science Department, University of Florida, Gainesville, Florida2
Received 11 October 2004/
Accepted 14 December 2004
Methylobacterium extorquens AM1 was used to explore the genetics of dephosphotetrahydromethanopterin (dH4MPT) biosynthesis. Strains with mutations in eight "archaeal-type" genes linked on the chromosome of M. extorquens AM1 were analyzed for the ability to synthesize dH4MPT, and six were found to be dH4MPT negative. Putative functions of these genes in dH4MPT biosynthesis are discussed.
* Corresponding author. Mailing address: 231 Wilcox Hall, Box 352125, University of Washington, Seattle, WA 98195. Phone: (206) 543-6683. Fax: (206) 616-5721. E-mail: milachis{at}u.washington.edu.
Journal of Bacteriology, April 2005, p. 2508-2512, Vol. 187, No. 7
0021-9193/05/$08.00+0 doi:10.1128/JB.187.7.2508-2512.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.
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Copyright © 2005 by the American Society for Microbiology. All rights reserved.