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Journal of Bacteriology, April 2005, p. 2526-2531, Vol. 187, No. 7
0021-9193/05/$08.00+0     doi:10.1128/JB.187.7.2526-2531.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Identification of Cohesive Ends and Genes Encoding the Terminase of Phage 16-3

Anita Ganyu,¹ Zsolt Csiszovszki,^1,2 Tamás Ponyi,² András Kern,^1, Zsuzsanna Buzás,¹ László Orosz,^1,2 and Péter P. Papp^1*

Institute of Genetics, Agricultural Biotechnology Center, Gödöllo",¹ Department of Genetics, Faculty of Science, Eötvös Loránd Science University and Research, Group for Molecular Genetics of the Hungarian Academy of Sciences, Budapest, Hungary²

Received 2 November 2004/ Accepted 3 January 2005

Cohesive ends of 16-3, a temperate phage of Rhizobium meliloti 41, have been identified as 10-base-long, 3'-protruding complementary G/C-rich sequences. terS and terL encode the two subunits of 16-3 terminase. Significant homologies were detected among the terminase subunits of phage 16-3 and other phages from various ecosystems.

Present address: Institute of Enzymology, Biological Research Centre, Hungarian Academy of Sciences, H-1113 Budapest, Hungary.

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