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Journal of Bacteriology, April 2005, p. 2618-2627, Vol. 187, No. 8
0021-9193/05/$08.00+0     doi:10.1128/JB.187.8.2618-2627.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

An FNR-Type Regulator Controls the Anaerobic Expression of Hyn Hydrogenase in Thiocapsa roseopersicina

Ákos T. Kovács,1,2 Gábor Rákhely,1 Douglas F. Browning,2 András Fülöp,1 Gergely Maróti,1 Stephen J. W. Busby,2 and Kornél L. Kovács1*

Department of Biotechnology, University of Szeged, and Institute of Biophysics, Biological Research Center of the Hungarian Academy of Sciences, Szeged, Hungary,1 School of Biosciences, University of Birmingham, Edgbaston, Birmingham, United Kingdom2

Received 6 October 2004/ Accepted 10 January 2005

The purple sulfur photosynthetic bacterium Thiocapsa roseopersicina BBS contains a heat-stable membrane-associated hydrogenase encoded by the hyn operon. Expression from the hyn operon regulatory region is up-regulated under anaerobic conditions. cis elements were mapped between positions –602 and –514 upstream from the hynS gene. Within this region two sequences that resemble DNA sites for FNR were recognized. The gene of an FNR homologue, FnrT, was identified in the genome of T. roseopersicina, and an fnrT knockout mutant was constructed. Anaerobic induction of hynS expression was abolished in the fnrT mutant, suggesting that FnrT is an activator of the hynS promoter. The T. roseopersicina hynS promoter could be activated in Escherichia coli, and this regulation was dependent on E. coli FNR. In vitro experiments with purified E. coli Ala154 FNR protein and purified E. coli RNA polymerase showed that FNR bound to two sites in the hyn regulatory region, that FNR could activate transcription initiation at the hynS promoter, and that FNR bound at the two target sites activated to different extents.

* Corresponding author. Mailing address: Department of Biotechnology, University of Szeged, H-6726 Szeged, Temesvári krt. 62, Hungary, Phone: 36 62 544351. Fax: 36 62 544352. E-mail: kornel{at}nucleus.szbk.u-szeged.hu.

Journal of Bacteriology, April 2005, p. 2618-2627, Vol. 187, No. 8
0021-9193/05/$08.00+0     doi:10.1128/JB.187.8.2618-2627.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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