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Journal of Bacteriology, April 2005, p. 2628-2637, Vol. 187, No. 8
0021-9193/05/$08.00+0     doi:10.1128/JB.187.8.2628-2637.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Flavobacterium johnsoniae GldJ Is a Lipoprotein That Is Required for Gliding Motility

Timothy F. Braun and Mark J. McBride*

Department of Biological Sciences, University of Wisconsin—Milwaukee, Milwaukee, Wisconsin

Received 13 December 2004/ Accepted 10 January 2005

Cells of Flavobacterium johnsoniae glide rapidly over surfaces by an unknown mechanism. Eight genes required for gliding motility have been described. Complementation of the nonmotile mutant UW102-48 identified another gene, gldJ, that is required for gliding. gldJ mutants formed nonspreading colonies, and individual cells were completely nonmotile. Like previously described nonmotile mutants, gldJ mutants were deficient in chitin utilization and were resistant to bacteriophages that infect wild-type cells. Cell fractionation and labeling studies with [3H]palmitate indicated that GldJ is a lipoprotein. Mutations in gldA, gldB, gldD, gldF, gldG, gldH, or gldI resulted in normal levels of gldJ transcript but decreased levels of GldJ protein. Expression of truncated GldJ protein in wild-type cells resulted in a severe motility defect. GldJ was found in regular bands that suggest the presence of a helical structure within the cell envelope.

* Corresponding author. Mailing address: Department of Biological Sciences, 181 Lapham Hall, University of Wisconsin—Milwaukee, 3209 N. Maryland Ave., Milwaukee, WI 53211. Phone: (414) 229-5844. Fax: (414) 229-3926. E-mail: mcbride{at}uwm.edu.

Journal of Bacteriology, April 2005, p. 2628-2637, Vol. 187, No. 8
0021-9193/05/$08.00+0     doi:10.1128/JB.187.8.2628-2637.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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