This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Grantcharova, N. Articles by Flärdh, K. Search for Related Content PubMed PubMed Citation Articles by Grantcharova, N. Articles by Flärdh, K.

Dynamics of FtsZ Assembly during Sporulation in Streptomyces coelicolor A3(2)

Department of Cell and Molecular Biology, Uppsala University, BMC Box 596, SE-751 24 Uppsala, Sweden

Received 15 September 2004/ Accepted 27 January 2005

FtsZ, the bacterial tubulin homologue, is the main player in at least two distinct processes of cell division during the development of Streptomyces coelicolor A3(2). It forms cytokinetic rings and is required for the formation of both the widely spaced hyphal cross walls in the substrate mycelium and the specialized septation that converts sporogenic aerial hyphae into spores. The latter developmentally controlled septation involves the coordinated assembly of large numbers of FtsZ rings in each sporulating hyphal cell. We used an FtsZ-enhanced green fluorescent protein (EGFP) translational fusion to visualize the progression of FtsZ ring assembly in vivo during sporulation of aerial hyphae. This revealed that the regular placement of multiple FtsZ rings and initiation of cytokinesis was preceded by a protracted phase during which spiral-shaped FtsZ intermediates were detected along the length of the aerial hyphal cell. Time course experiments indicated that they were remodeled and gradually replaced by regularly spaced FtsZ rings. Such spiral-shaped filaments could also be detected with immunofluorescence microscopy using an antiserum against FtsZ. Based on our observations, we propose a model for the progression of Z-ring assembly during sporulation of S. coelicolor. Furthermore, mutants lacking the developmental regulatory genes whiA, whiB, whiG, whiH, and whiI were investigated. They failed in up-regulation of the expression of FtsZ-EGFP in aerial hyphae, which is consistent with the known effects of these genes on ftsZ transcription.

This article has been cited by other articles:

• Hett, E. C., Rubin, E. J. (2008). Bacterial Growth and Cell Division: a Mycobacterial Perspective. Microbiol. Mol. Biol. Rev. 72: 126-156 [Abstract] [Full Text]  
• Craney, A., Hohenauer, T., Xu, Y., Navani, N. K., Li, Y., Nodwell, J. (2007). A synthetic luxCDABE gene cluster optimized for expression in high-GC bacteria. Nucleic Acids Res 35: e46-e46 [Abstract] [Full Text]  
• Wang, L., Yu, Y., He, X., Zhou, X., Deng, Z., Chater, K. F., Tao, M. (2007). Role of an FtsK-Like Protein in Genetic Stability in Streptomyces coelicolor A3(2). J. Bacteriol. 189: 2310-2318 [Abstract] [Full Text]  
• Carballido-Lopez, R. (2006). The Bacterial Actin-Like Cytoskeleton. Microbiol. Mol. Biol. Rev. 70: 888-909 [Abstract] [Full Text]  
• Michie, K. A., Monahan, L. G., Beech, P. L., Harry, E. J. (2006). Trapping of a Spiral-Like Intermediate of the Bacterial Cytokinetic Protein FtsZ.. J. Bacteriol. 188: 1680-1690 [Abstract] [Full Text]  
• Chauhan, A., Madiraju, M. V. V. S., Fol, M., Lofton, H., Maloney, E., Reynolds, R., Rajagopalan, M. (2006). Mycobacterium tuberculosis Cells Growing in Macrophages Are Filamentous and Deficient in FtsZ Rings.. J. Bacteriol. 188: 1856-1865 [Abstract] [Full Text]  
• Del Sol, R., Mullins, J. G. L., Grantcharova, N., Flardh, K., Dyson, P. (2006). Influence of CrgA on Assembly of the Cell Division Protein FtsZ during Development of Streptomyces coelicolor. J. Bacteriol. 188: 1540-1550 [Abstract] [Full Text]