This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Peña-Sandoval, G. R.
Right arrow Articles by Georgellis, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Peña-Sandoval, G. R.
Right arrow Articles by Georgellis, D.

 Previous Article  |  Next Article 

Journal of Bacteriology, May 2005, p. 3267-3272, Vol. 187, No. 9
0021-9193/05/$08.00+0     doi:10.1128/JB.187.9.3267-3272.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Requirement of the Receiver and Phosphotransfer Domains of ArcB for Efficient Dephosphorylation of Phosphorylated ArcA In Vivo

Gabriela R. Peña-Sandoval,1 Ohsuk Kwon,2 and Dimitris Georgellis1*

Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, 04510 Mexico City, Mexico,1 Laboratory of Metabolic Engineering, Korea Research Institute of Bioscience and Biotechnology, 52 Oun-dong, Yusong-gu, Daejeon 305-333, Korea2

Received 29 October 2004/ Accepted 21 January 2005

The Arc two-component system, comprising the ArcB sensor kinase and the ArcA response regulator, modulates the expression of numerous genes in response to the respiratory conditions of growth. Under anoxic growth conditions, ArcB autophosphorylates and transphosphorylates ArcA, which in turn represses or activates its target operons. Under aerobic growth conditions, phosphorylated ArcA (ArcA-P) dephosphorylates and its transcriptional regulation is released. The dephosphorylation of ArcA-P has been shown to occur, at least in vitro, via an ArcAAsp54-P -> ArcBHis717-P -> ArcBAsp576-P -> Pi reverse phosphorelay. In this study, the physiological significance of this pathway was assessed. The results demonstrate that the receiver and phosphotransfer domains of the tripartite sensor kinase ArcB are necessary and sufficient for efficient ArcA-P dephosphorylation in vivo.

* Corresponding author. Mailing address: Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, 04510 Mexico City, Mexico. Phone: 52 55 5622 5738. Fax: 52 55 5622 5611. E-mail: dimitris{at}ifc.unam.mx.

Journal of Bacteriology, May 2005, p. 3267-3272, Vol. 187, No. 9
0021-9193/05/$08.00+0     doi:10.1128/JB.187.9.3267-3272.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Whitworth, D. E., Cock, P. J. A. (2008). Two-component systems of the myxobacteria: structure, diversity and evolutionary relationships. Microbiology 154: 360-372 [Abstract] [Full Text]  
  • To, J. P.C., Deruere, J., Maxwell, B. B., Morris, V. F., Hutchison, C. E., Ferreira, F. J., Schaller, G. E., Kieber, J. J. (2007). Cytokinin Regulates Type-A Arabidopsis Response Regulator Activity and Protein Stability via Two-Component Phosphorelay. Plant Cell 19: 3901-3914 [Abstract] [Full Text]  
  • Mika, F., Hengge, R. (2005). A two-component phosphotransfer network involving ArcB, ArcA, and RssB coordinates synthesis and proteolysis of {sigma}S (RpoS) in E. coli. Genes Dev. 19: 2770-2781 [Abstract] [Full Text]  
  • Tomenius, H., Pernestig, A.-K., Mendez-Catala, C. F., Georgellis, D., Normark, S., Melefors, O. (2005). Genetic and Functional Characterization of the Escherichia coli BarA-UvrY Two-Component System: Point Mutations in the HAMP Linker of the BarA Sensor Give a Dominant-Negative Phenotype. J. Bacteriol. 187: 7317-7324 [Abstract] [Full Text]  
  • Majdalani, N., Heck, M., Stout, V., Gottesman, S. (2005). Role of RcsF in Signaling to the Rcs Phosphorelay Pathway in Escherichia coli. J. Bacteriol. 187: 6770-6778 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»