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Multistress Regulation in Escherichia coli: Expression of osmB Involves Two Independent Promoters Responding either to ^S or to the RcsCDB His-Asp Phosphorelay

Alice Boulanger, Anne Francez-Charlot, Annie Conter, Marie-Pierre Castanié-Cornet, Kaymeuang Cam, and Claude Gutierrez^*

Laboratoire de Microbiologie et Génétique Moléculaires, UMR5100 Centre National de la Recherche Scientifique-Université Toulouse III, 31062 Toulouse, France

Received 18 November 2004/ Accepted 22 January 2005

Transcription of the Escherichia coli osmB gene is induced by several stress conditions. osmB is expressed from two promoters, osmBp1 and osmBp2. The downstream promoter, osmBp2, is induced after osmotic shock or upon entry into stationary phase in a ^S-dependent manner. The upstream promoter, osmBp1, is independent of ^S and is activated by RcsB, the response regulator of the His-Asp phosphorelay signal transduction system RcsCDB. RcsB is responsible for the induction of osmBp1 following treatment with chlorpromazine. Activation of osmBp1 by RcsB requires a sequence upstream of its –35 element similar to the RcsB binding site consensus, suggesting a direct regulatory role. osmB appears as another example of a multistress-responsive gene whose transcription involves both a ^S-dependent promoter and a second one independent of ^S but controlled by stress-specific transcription factors.

Present address: Centre de Biologie du Développement, 118 route de Narbonne, 31062 Toulouse, France.

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