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Journal of Bacteriology, January 2006, p. 297-304, Vol. 188, No. 1
0021-9193/06/$08.00+0     doi:10.1128/JB.188.1.297-304.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

A Little Gene with Big Effects: a serT Mutant Is Defective in flgM Gene Translation

Fabienne F. V. Chevance,1 Joyce E. Karlinsey,2 Christopher E. Wozniak,1,2 and Kelly T. Hughes1*

Department of Biology, University of Utah, Salt Lake City, Utah 84112,1 Department of Microbiology, Box 357242, University of Washington, Seattle, Washington 981952

Received 26 August 2005/ Accepted 29 September 2005

A conditional-lethal mutant was isolated as having a flagellar regulatory phenotype at 30°C and being unable to grow at 42°C. Chromosomal mapping localized the mutation to the serT gene, which encodes an essential serine tRNA species (). DNA sequence analysis revealed the mutation to be a single base change in G:A at position 10 of the serT gene that lies within the D-stem of the essential species. recognizes UCA, UCG, and UCU codons, but UCU is also recognized by and UCG by . No other tRNAs are known to read the UCA codon. Thus, the UCA codon is specifically recognized by . We show that the anti-{sigma}28 activity of FlgM is defective in the serT mutant strain. The serT allele causes a 10-fold increase in {sigma}28-dependent fliC promoter transcription, indicating a defect in FlgM anti-{sigma}28 activity in the presence of the serT mutation. The flgM gene contains only one UCA codon. Changing the UCA of flgM to ACG reversed the effect of the serT allele. Implications for context effects in regulation of gene expression are discussed.

* Corresponding author. Mailing address: Department of Biology, University of Utah, Salt Lake City, UT 84112. Phone: (801) 581-6517. Fax: (801) 581-4668. E-mail: hughes{at}biology.utah.edu.

Journal of Bacteriology, January 2006, p. 297-304, Vol. 188, No. 1
0021-9193/06/$08.00+0     doi:10.1128/JB.188.1.297-304.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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  • Chevance, F. F.V., Takahashi, N., Karlinsey, J. E., Gnerer, J., Hirano, T., Samudrala, R., Aizawa, S.-I., Hughes, K. T. (2007). The mechanism of outer membrane penetration by the eubacterial flagellum and implications for spirochete evolution. Genes Dev. 21: 2326-2335 [Abstract] [Full Text]  


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