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Journal of Bacteriology, January 2006, p. 343-347, Vol. 188, No. 1
0021-9193/06/$08.00+0     doi:10.1128/JB.188.1.343-347.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Disruption of sucA, Which Encodes the E1 Subunit of {alpha}-Ketoglutarate Dehydrogenase, Affects the Survival of Nitrosomonas europaea in Stationary Phase

Norman G. Hommes, Elizabeth G. Kurth, Luis A. Sayavedra-Soto, and Daniel J. Arp*

Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon 97331-2902

Received 27 July 2005/ Accepted 6 October 2005

Although Nitrosomonas europaea lacks measurable {alpha}-ketoglutarate dehydrogenase activity, the recent completion of the genome sequence revealed the presence of the genes encoding the enzyme. A knockout mutation was created in the sucA gene encoding the E1 subunit. Compared to wild-type cells, the mutant strain showed an accelerated loss of ammonia monooxygenase and hydroxylamine oxidoreductase activities upon entering stationary phase. In addition, unlike wild-type cells, the mutant strain showed a marked lag in the ability to resume growth in response to pH adjustments in late stationary phase.

* Corresponding author. Mailing address: Department of Botany and Plant Pathology, Oregon State University, 2082 Cordley, Corvallis, OR 97331-2902. Phone: (541) 737-1294. Fax: (541) 737-5310. E-mail: arpd{at}science.oregonstate.edu.

Journal of Bacteriology, January 2006, p. 343-347, Vol. 188, No. 1
0021-9193/06/$08.00+0     doi:10.1128/JB.188.1.343-347.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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