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Journal of Bacteriology, January 2006, p. 55-63, Vol. 188, No. 1
0021-9193/06/$08.00+0     doi:10.1128/JB.188.1.55-63.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Identification of the Lower Baseplate Protein as the Antireceptor of the Temperate Lactococcal Bacteriophages TP901-1 and Tuc2009

Christina S. Vegge,1,{dagger} Finn K. Vogensen,1 Stephen Mc Grath,2 Horst Neve,3 Douwe van Sinderen,2,4 and Lone Brøndsted5*

Department of Food Science, The Royal Veterinary and Agricultural University, Frederiksberg C, Denmark,1 National Food Biotechnology Centre and Department of Microbiology, National University of Ireland, Cork, Ireland,2 Institute for Microbiology, Federal Research Centre for Nutrition and Food, Kiel, Germany,3 Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland,4 Department of Veterinary Pathobiology, The Royal Veterinary and Agricultural University, Frederiksberg C, Denmark5

Received 31 May 2005/ Accepted 7 October 2005

The first step in the infection process of tailed phages is recognition and binding to the host receptor. This interaction is mediated by the phage antireceptor located in the distal tail structure. The temperate Lactococcus lactis phage TP901-1 belongs to the P335 species of the Siphoviridae family, which also includes the related phage Tuc2009. The distal tail structure of TP901-1 is well characterized and contains a double-disk baseplate and a central tail fiber. The structural tail proteins of TP901-1 and Tuc2009 are highly similar, but the phages have different host ranges and must therefore encode different antireceptors. In order to identify the antireceptors of TP901-1 and Tuc2009, a chimeric phage was generated in which the gene encoding the TP901-1 lower baseplate protein (bppLTP901-1) was exchanged with the analogous gene (orf532009) of phage Tuc2009. The chimeric phage (TP901-1C) infected the Tuc2009 host strain efficiently and thus displayed an altered host range compared to TP901-1. Genomic analysis and sequencing verified that TP901-1C is a TP901-1 derivative containing the orf532009 gene in exchange for bppLTP901-1; however, a new sequence in the late promoter region was also discovered. Protein analysis confirmed that TP901-1C contains ORF532009 and not the lower baseplate protein BppLTP901-1, and it was concluded that BppLTP901-1 and ORF532009 constitute antireceptor proteins of TP901-1 and Tuc2009, respectively. Electron micrographs revealed altered baseplate morphology of TP901-1C compared to that of the parental phage.

* Corresponding author. Mailing address: Department of Veterinary Pathobiology, The Royal Veterinary and Agricultural University, Stigbøjlen 4, DK-1870 Frederiksberg C, Denmark. Phone: 45 35 28 27 64. Fax: 45 35 28 2757. E-mail: lobr{at}kvl.dk.

{dagger} Present address: Department of Veterinary Pathobiology, The Royal Veterinary and Agricultural University, Frederiksberg C, Denmark.

Journal of Bacteriology, January 2006, p. 55-63, Vol. 188, No. 1
0021-9193/06/$08.00+0     doi:10.1128/JB.188.1.55-63.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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