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Journal of Bacteriology, May 2006, p. 3525-3534, Vol. 188, No. 10
0021-9193/06/$08.00+0     doi:10.1128/JB.188.10.3525-3534.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Characterization of the Yersinia enterocolitica Type III Secretion ATPase YscN and Its Regulator, YscL

Bill Blaylock, Kelly E. Riordan, Dominique M. Missiakas, and Olaf Schneewind*

Department of Microbiology, University of Chicago, Chicago, Illinois 60637

Received 8 December 2005/ Accepted 3 March 2006

Type III secretion is a mechanism used by a broad range of gram-negative bacteria to neutralize eukaryotic defenses by enabling translocation of bacterial proteins directly into the cytoplasm of host cells. The bacterial energy source for secretion is ATP, which is consumed by an ATPase that couples ATP hydrolysis to the unfolding of secreted proteins and the dissociation of their chaperones just prior to secretion. By studying the biochemical properties of YscN and YscL of Yersinia enterocolitica, we have characterized them as the ATPase and ATPase regulator, respectively, of the type III secretion system of this organism. In vivo, YscL and YscN interact with each other, and the overexpression of glutathione S-transferase-YscL abolishes secretion and down-regulates the expression of secretion apparatus components.

* Corresponding author. Mailing address: Department of Microbiology, University of Chicago, CLSC607B, 920 East 58th Street, Chicago, IL 60627. Phone: (773) 834-9060. Fax: (773) 834-8150. E-mail: oschnee{at}bsd.uchicago.edu.

Journal of Bacteriology, May 2006, p. 3525-3534, Vol. 188, No. 10
0021-9193/06/$08.00+0     doi:10.1128/JB.188.10.3525-3534.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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