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Journal of Bacteriology, May 2006, p. 3600-3613, Vol. 188, No. 10
0021-9193/06/$08.00+0     doi:10.1128/JB.188.10.3600-3613.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Transcriptome and Phenotypic Responses of Vibrio cholerae to Increased Cyclic di-GMP Level{dagger}

Sinem Beyhan,1 Anna D. Tischler,2 Andrew Camilli,2 and Fitnat H. Yildiz1*

Department of Environmental Toxicology, University of California, Santa Cruz, 269 Jack Baskin Engineering Bldg., Santa Cruz, California 95064,1 Howard Hughes Medical Institute and Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 136 Harrison Avenue, Boston, Massachusetts 021112

Received 10 November 2005/ Accepted 15 February 2006

Vibrio cholerae, the causative agent of cholera, is a facultative human pathogen with intestinal and aquatic life cycles. The capacity of V. cholerae to recognize and respond to fluctuating parameters in its environment is critical to its survival. In many microorganisms, the second messenger, 3',5'-cyclic diguanylic acid (c-di-GMP), is believed to be important for integrating environmental stimuli that affect cell physiology. Sequence analysis of the V. cholerae genome has revealed an abundance of genes encoding proteins with either GGDEF domains, EAL domains, or both, which are predicted to modulate cellular c-di-GMP concentrations. To elucidate the cellular processes controlled by c-di-GMP, whole-genome transcriptome responses of the El Tor and classical V. cholerae biotypes to increased c-di-GMP concentrations were determined. The results suggest that V. cholerae responds to an elevated level of c-di-GMP by increasing the transcription of the vps, eps, and msh genes and decreasing that of flagellar genes. The functions of other c-di-GMP-regulated genes in V. cholerae are yet to be identified.

* Corresponding author. Mailing address: Department of Environmental Toxicology, University of California, Santa Cruz, 269 Jack Baskin Engineering Bldg., Santa Cruz, CA 95064. Phone: (831) 459-1588. Fax: (831) 459-3524. E-mail: yildiz{at}etox.ucsc.edu.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, May 2006, p. 3600-3613, Vol. 188, No. 10
0021-9193/06/$08.00+0     doi:10.1128/JB.188.10.3600-3613.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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