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Journal of Bacteriology, June 2006, p. 3748-3756, Vol. 188, No. 11
0021-9193/06/$08.00+0     doi:10.1128/JB.00169-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Different Roles of EIIABMan and EIIGlc in Regulation of Energy Metabolism, Biofilm Development, and Competence in Streptococcus mutans

Jacqueline Abranches,1 Melissa M. Candella,1 Zezhang T. Wen,1 Henry V. Baker,2 and Robert A. Burne1*

Department of Oral Biology, University of Florida College of Dentistry, P.O. Box 100424, Gainesville, Florida 32610,1 Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, Florida 326012

Received 31 January 2006/ Accepted 21 March 2006

The phosphoenolpyruvate:sugar phosphotransferase system (PTS) is the major carbohydrate transport system in oral streptococci. The mannose-PTS of Streptococcus mutans, which transports mannose and glucose, is involved in carbon catabolite repression (CCR) and regulates the expression of known virulence genes. In this study, we investigated the role of EIIGlc and EIIABMan in sugar metabolism, gene regulation, biofilm formation, and competence. The results demonstrate that the inactivation of ptsG, encoding a putative EIIGlc, did not lead to major changes in sugar metabolism or affect the phenotypes of interest. However, the loss of EIIGlc was shown to have a significant impact on the proteome and to affect the expression of a known virulence factor, fructan hydrolase (fruA). JAM1, a mutant strain lacking EIIABMan, had an impaired capacity to form biofilms in the presence of glucose and displayed a decreased ability to be transformed with exogenous DNA. Also, the lactose- and cellobiose-PTSs were positively and negatively regulated by EIIABMan, respectively. Microarrays were used to investigate the profound phenotypic changes displayed by JAM1, revealing that EIIABMan of S. mutans has a key regulatory role in energy metabolism, possibly by sensing the energy levels of the cells or the carbohydrate availability and, in response, regulating the activity of transcription factors and carbohydrate transporters.

* Corresponding author. Mailing address: Department of Oral Biology, University of Florida College of Dentistry, P.O. Box 100424, Gainesville, FL 32610. Phone: (352) 392-4370. Fax: (352) 392-7357. E-mail: rburne{at}dental.ufl.edu.

Journal of Bacteriology, June 2006, p. 3748-3756, Vol. 188, No. 11
0021-9193/06/$08.00+0     doi:10.1128/JB.00169-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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