JB
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mc Grath, S.
Right arrow Articles by van Sinderen, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mc Grath, S.
Right arrow Articles by van Sinderen, D.

 Previous Article  |  Next Article 

Journal of Bacteriology, June 2006, p. 3972-3982, Vol. 188, No. 11
0021-9193/06/$08.00+0     doi:10.1128/JB.00024-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Anatomy of a Lactococcal Phage Tail{dagger}

Stephen Mc Grath,1* Horst Neve,2 Jos F. M. L. Seegers,3 Robyn Eijlander,4 Christina S. Vegge,5 Lone Brøndsted,5 Knut J. Heller,2 Gerald F. Fitzgerald,1,7 Finn K. Vogensen,6 and Douwe van Sinderen1,7

Department of Microbiology, National University of Ireland, Cork, Ireland,1 Institute for Microbiology, Federal Research Centre for Nutrition and Food, Kiel, Germany,2 Lactrys Biopharmaceuticals, Zernikedreef 9, 2333 CK Leiden, The Netherlands,3 Department of Microbiology and Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands,4 Department of Veterinary Pathobiology, The Royal Veterinary and Agricultural University, Frederiksberg C, Denmark,5 Department of Food Science, The Royal Veterinary and Agricultural University, Frederiksberg C, Denmark,6 Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland7

Received 9 January 2006/ Accepted 10 March 2006

Bacteriophages of the Siphoviridae family utilize a long noncontractile tail to recognize, adsorb to, and inject DNA into their bacterial host. The tail anatomy of the archetypal Siphoviridae {lambda} has been well studied, in contrast to phages infecting gram-positive bacteria. This report outlines a detailed anatomical description of a typical member of the Siphoviridae infecting a gram-positive bacterium. The tail superstructure of the lactococcal phage Tuc2009 was investigated using N-terminal protein sequencing, Western blotting, and immunogold transmission electron microscopy, allowing a tangible path to be followed from gene sequence through encoded protein to specific architectural structures on the Tuc2009 virion. This phage displays a striking parity with {lambda} with respect to tail structure, which reenforced a model proposed for Tuc2009 tail architecture. Furthermore, comparisons with {lambda} and other lactococcal phages allowed the specification of a number of genetic submodules likely to encode specific tail structures.

* Corresponding author. Mailing address: Department of Microbiology, National University of Ireland, Cork, Ireland. Phone: 353 21 4903146. Fax: 353 21 4903101. E-mail: smcgrath{at}ucc.ie.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, June 2006, p. 3972-3982, Vol. 188, No. 11
0021-9193/06/$08.00+0     doi:10.1128/JB.00024-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Appl. Environ. Microbiol. Infect. Immun. Eukaryot. Cell
Mol. Cell. Biol. J. Virol. Microbiol. Mol. Biol. Rev.
ALL ASM JOURNALS

Copyright © 2006 by the American Society for Microbiology. All rights reserved.