This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Perez, E.
Right arrow Articles by Stock, A. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Perez, E.
Right arrow Articles by Stock, A. M.

 Previous Article  |  Next Article 

Journal of Bacteriology, June 2006, p. 4093-4100, Vol. 188, No. 11
0021-9193/06/$08.00+0     doi:10.1128/JB.00181-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Identification of Methylation Sites in Thermotoga maritima Chemotaxis Receptors

Eduardo Perez,1,2 Haiyan Zheng,1,3 and Ann M. Stock1,2,4*

Center for Advanced Biotechnology and Medicine,1 Department of Biochemistry,2 Department of Pharmacology,3 Howard Hughes Medical Institute, UMDNJ-Robert Wood Johnson Medical School, Piscataway, New Jersey 088544

Received 3 February 2006/ Accepted 17 March 2006

Adaptation in bacterial chemotaxis involves reversible methylation of specific glutamate residues within the cytoplasmic domains of methyl-accepting chemotaxis proteins. The specific sites of methylation in Salmonella enterica and Escherichia coli chemoreceptors, identified 2 decades ago, established a consensus sequence for methylation by methyltransferase CheR. Here we report the in vitro methylation of chemoreceptors from Thermotoga maritima, a hyperthermophile that has served as a useful source of chemotaxis proteins for structural analysis. Sites of methylation have been identified by liquid chromatography-mass spectrometry/mass spectrometry. Fifteen sites of methylation were identified within the cytoplasmic domains of four different T. maritima chemoreceptors. The results establish a consensus sequence for chemoreceptor methylation sites in T. maritima that is distinct from the previously identified consensus sequence for E. coli and S. enterica. These findings suggest that consensus sequences for posttranslational modifications in one organism may not be directly extrapolated to analogous modifications in other bacteria.

* Corresponding author. Mailing address: CABM, 679 Hoes Lane, Piscataway, NJ 08854-5627. Phone: (732) 235-4844. Fax: (732) 235-5289. E-mail: stock{at}cabm.rutgers.edu.

Journal of Bacteriology, June 2006, p. 4093-4100, Vol. 188, No. 11
0021-9193/06/$08.00+0     doi:10.1128/JB.00181-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Alexander, R. P., Zhulin, I. B. (2007). From the Cover: Evolutionary genomics reveals conserved structural determinants of signaling and adaptation in microbial chemoreceptors. Proc. Natl. Acad. Sci. USA 104: 2885-2890 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»